Description

6-Chloro-3-Indolyl-β-D-Galactopyranoside is a chromogenic substrate widely used in molecular biology and biochemistry for the detection of β-galactosidase activity. This compound is critical for applications such as blue-white screening in molecular cloning, reporter gene assays, and histochemical staining. It is an essential reagent for research and development laboratories in the biotechnology, pharmaceutical, and life sciences sectors.

Application

• Blue-White Screening in Molecular Cloning: Used as a substrate to identify recombinant bacterial colonies containing plasmids with inserts, where β-galactosidase activity is disrupted.
• Reporter Gene Assays: Employed to measure and quantify gene expression levels in cell-based assays where the β-galactosidase gene is used as a reporter.
• Histochemistry & Cytochemistry: Applied in tissue and cell staining protocols to localize β-galactosidase enzyme activity, producing an insoluble blue precipitate.
• Enzyme Activity Studies: Serves as a specific substrate for kinetic studies and characterization of β-galactosidase enzymes from various sources.
• Diagnostic Kit Manufacturing: Incorporated as a key component in diagnostic kits for detecting specific pathogens or genetic markers via enzymatic reactions.
• Microbiology Research: Used in studies of bacterial gene regulation and promoter activity where lacZ or similar genes are involved.

Basic Information

Product Name	6-Chloro-3-Indolyl-β-D-Galactopyranoside
CAS No.	159954-28-6
Molecular Formula	C20H24ClNO11
Molecular Weight	489.86 g/mol
Synonyms	6-Chloro-3-indolyl β-D-galactopyranoside; 6-Chloro-3-indolyl-β-D-galactoside; 6-Cl-β-Gal; 6-Chloroindoxyl-β-D-galactopyranoside; X-Gal (6-Chloro derivative); 6-Chloro-1H-indol-3-yl β-D-galactopyranoside; 6-Chloro-3-indolyl-β-galactoside; 5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside analog
EINECS	Contact for details

Quality Control

Our 6-Chloro-3-Indolyl-β-D-Galactopyranoside is manufactured under strict quality systems to ensure high purity and batch-to-batch consistency suitable for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard enzymatic assays. We adhere to ISO 9001 quality management principles, and our production environment is designed to meet the stringent requirements of the life sciences industry.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. The product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to ambient conditions or light may lead to degradation. For long-term stability, store desiccated under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference standard
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMF, DMSO; slightly soluble in water
Melting Point	Contact for details
Heavy Metals	< 10 ppm
Residual Solvents (GC)	Meets ICH guidelines
Biological Activity	Produces characteristic blue color with β-galactosidase

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.