Description

Uridine, 5'-o-[Bis(4-Methoxyphenyl)Phenylmethyl]-2'-o-[(1,1-Dimethylethyl)Dimethylsilyl]-5-Methyl-, 3'-[2-Cyanoethyl N,n-Bis(1-Methylethyl)Phosphoramidite] is a highly specialized, protected nucleoside phosphoramidite monomer, a critical building block in the automated solid-phase synthesis of oligonucleotides. This compound is engineered to provide exceptional coupling efficiency and stability during the synthesis of modified RNA sequences, particularly those containing 5-methyluridine. It is an essential raw material for research institutions and pharmaceutical companies developing advanced therapeutics, including antisense oligonucleotides, siRNAs, and mRNA-based vaccines and drugs.

Application

• Oligonucleotide Synthesis: Primary monomer for the automated solid-phase synthesis of RNA and modified RNA strands.
• Therapeutic RNA Development: Key raw material in the production of siRNA, antisense oligonucleotides (ASOs), and mRNA for therapeutic applications.
• Research & Diagnostics: Used in academic and industrial labs to synthesize probes, primers, and standards for genetic research and diagnostic assays.
• Synthesis of Modified Nucleosides: Enables the site-specific incorporation of 5-methyluridine into oligonucleotides to study structure-activity relationships and enhance nuclease resistance.
• Pre-clinical & Process Development: Supports scale-up activities from milligram to kilogram scales for pre-clinical and clinical trial material manufacturing.

Basic Information

Item	Detail
Product Name	Uridine, 5'-o-[Bis(4-Methoxyphenyl)Phenylmethyl]-2'-o-[(1,1-Dimethylethyl)Dimethylsilyl]-5-Methyl-, 3'-[2-Cyanoethyl N,n-Bis(1-Methylethyl)Phosphoramidite]
CAS No.	159639-78-8
Molecular Formula	C48H64N3O8PSi
Molecular Weight	878.10 g/mol
Synonyms	5'-O-DMT-2'-O-TBDMS-5-methyluridine-3'-CE phosphoramidite; 5-Methyluridine, 5'-O-(bis(4-methoxyphenyl)phenylmethyl)-2'-O-[(1,1-dimethylethyl)dimethylsilyl]-, 3'-[2-cyanoethyl N,N-diisopropylphosphoramidite]; 5-Me-U Phosphoramidite (DMT, 2'-TBDMS); 2'-O-TBDMS-5-methyluridine-3'-phosphoramidite; 5-Methyluridine CE Phosphoramidite; RNA Phosphoramidite Monomer (5-MeU); N/A
EINECS	Contact for details

Quality Control

Every batch of this high-purity phosphoramidite is manufactured under strict quality management systems and undergoes comprehensive analytical characterization. Quality is assured through multiple orthogonal techniques including HPLC, 31P NMR, and 1H NMR to confirm identity, purity, and reactivity. A Certificate of Analysis (COA) detailing all specifications is provided with each shipment. Our production adheres to cGMP guidelines for active pharmaceutical ingredients (APIs) where required, ensuring suitability for therapeutic development.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. This compound is hygroscopic (moisture-sensitive) and easily oxidized. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation. For long-term stability, store desiccated at -20°C.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
Assay (31P NMR)	≥ 97.0%
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.