Description

n-(4-Maleimidobutyryloxy)Sulfosuccinimide Sodium Salt (Sulfo-GMBS) is a heterobifunctional crosslinking reagent featuring a water-soluble sulfo-NHS ester and a maleimide group. This compound is essential for creating stable, site-specific conjugates between amine-containing molecules and sulfhydryl groups under mild, aqueous conditions. It is a critical tool for researchers and manufacturers in the bioconjugation, diagnostics, and therapeutic development sectors who require efficient and reliable crosslinking with enhanced solubility.

Application

• Antibody-Drug Conjugate (ADC) Development: Site-specific conjugation of cytotoxic drugs to monoclonal antibodies via cysteine residues.
• Protein and Peptide Labeling: Covalent attachment of fluorescent dyes, enzymes (e.g., HRP), or biotin to proteins for assays and imaging.
• Immobilization Chemistry: Functionalization of amine-modified surfaces (e.g., chips, beads, plates) with thiol-containing ligands or capture molecules.
• Diagnostic Reagent Manufacturing: Conjugation of enzymes or other reporters to antibodies or antigens for ELISA and lateral flow assays.
• Polymer Modification: Introduction of reactive maleimide groups into amine-functionalized polymers for further bioconjugation.
• Stable Conjugate Formation: Creating defined, non-cleavable linkages in vaccine candidates or biosensor components.

Basic Information

Product Name	n-(4-Maleimidobutyryloxy)Sulfosuccinimide Sodium Salt (Sulfo-GMBS)
CAS No.	158018-86-1
Molecular Formula	C16H13N2NaO11S
Molecular Weight	464.34 g/mol
Synonyms	Sulfo-GMBS; GMBS Sulfonate Sodium Salt; 4-Maleimidobutyric Acid Sulfosuccinimidyl Ester Sodium Salt; Sulfo-MBS; Sodium 4-[(2,5-Dioxopyrrol-1-yl)oxy]-4-oxobutyl-3-sulfonatosuccinate; N-(γ-Maleimidobutyryloxy)sulfosuccinimide Sodium Salt; 4-(N-Maleimidomethyl)cyclohexane-1-carboxylic acid 3-sulfo-N-hydroxysuccinimide ester sodium salt (common mis-association); Sulfo-EMCS analog
EINECS	Contact for details

Quality Control

Our n-(4-Maleimidobutyryloxy)Sulfosuccinimide Sodium Salt is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and NMR for structural confirmation, to ensure it meets the high standards required for sensitive bioconjugation applications. A detailed Certificate of Analysis (COA) with lot-specific data is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Solubility (Water)	Clear, colorless solution at 10 mg/mL

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.