Description

Z-Leu-Arg-7-Amino-4-Methylcoumarin is a specialized fluorogenic peptide substrate designed for high-sensitivity enzymatic assays. This compound is critical for researchers and quality control laboratories requiring precise measurement of protease activity, particularly trypsin and related serine proteases. It is primarily utilized in the pharmaceutical, biotechnology, and diagnostic industries for enzyme kinetics studies, inhibitor screening, and biochemical research.

Application

• Protease Activity Assays: As a fluorogenic substrate for the continuous, real-time monitoring of trypsin, thrombin, and other serine proteases.
• Drug Discovery & Development: High-throughput screening (HTS) of protease inhibitors for therapeutic target validation.
• Biochemical Research: Kinetic analysis and characterization of enzyme mechanisms and specificity.
• Diagnostic Reagent Manufacturing: A key component in the formulation of diagnostic kits for clinical and research use.
• Quality Control in Biologics: Monitoring protease activity and contamination in biopharmaceutical production processes.
• Academic Research: Fundamental studies in enzymology, cell biology, and proteolytic pathways.

Basic Information

Product Name	Z-Leu-Arg-7-Amino-4-Methylcoumarin
CAS No.	156192-32-4
Molecular Formula	C30H38N6O7
Molecular Weight	594.66 g/mol
Synonyms	Z-Leu-Arg-AMC; Benzyloxycarbonyl-Leucyl-Arginyl-7-Amino-4-Methylcoumarin; Z-Leu-Arg-AMC Hydrochloride (if applicable); Boc-Leu-Arg-AMC (related analog); Fluorogenic Trypsin Substrate; AMC-based Protease Substrate; Peptide-AMC Conjugate; CBZ-Leu-Arg-AMC
EINECS	Contact for details

Quality Control

Our Z-Leu-Arg-7-Amino-4-Methylcoumarin is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, mass spectrometry for identity confirmation, and spectrophotometry for performance validation. Certificates of Analysis (COA) with detailed chromatograms and spectral data are provided to ensure traceability and compliance with research-grade specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Fluorogenic Activity	Functional in standard trypsin assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.