Description

6-Chloro-3-Indoxyl-n-Acetyl-β-D-Glucosaminide is a specialized chromogenic substrate used for the enzymatic detection of N-acetyl-β-D-glucosaminidase (NAGase). This compound is critical for researchers and diagnostic manufacturers requiring high sensitivity and specificity in biochemical assays. It is primarily utilized in life science research, clinical diagnostics, and pharmaceutical development for applications such as enzyme activity studies and biomarker detection.

Application

• Clinical Diagnostic Assays: As a key substrate for detecting NAGase activity in urine, a biomarker for renal tubular damage and certain lysosomal storage disorders.
• Biochemical Research: For the study of glycosidase enzyme kinetics, inhibition, and function in various biological pathways.
• Pharmaceutical Screening: Used in high-throughput screening (HTS) platforms to identify potential inhibitors or activators of N-acetyl-β-D-glucosaminidase.
• Microbiology: Employed in culture media for the specific detection of bacterial species expressing relevant glycosidase enzymes.
• Histochemistry & Cell Biology: As a staining reagent for the localization of enzyme activity in tissue sections and cell cultures.
• Quality Control in Diagnostic Manufacturing: For the calibration and validation of diagnostic kits and automated analyzers that measure NAGase levels.

Basic Information

Item	Detail
Product Name	6-Chloro-3-Indoxyl-n-Acetyl-β-D-Glucosaminide
CAS No.	156117-44-1
Molecular Formula	C16H19ClN2O7
Molecular Weight	386.78 g/mol
Synonyms	6-Chloro-3-indolyl N-acetyl-β-D-glucosaminide; 6-Cl-3-Indoxyl NAG; NAG-6Cl; 6-Chloro-3-indoxyl 2-acetamido-2-deoxy-β-D-glucopyranoside; 2-Acetamido-2-deoxy-β-D-glucopyranosyl 6-chloro-3-indolyl ether; Chromogenic NAG substrate; β-GlcNAc-6Cl-3I; 156117-44-1
EINECS	Contact for details

Quality Control

Our 6-Chloro-3-Indoxyl-n-Acetyl-β-D-Glucosaminide is manufactured under strict quality systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets the stringent requirements for diagnostic and research applications. Certificates of Analysis (COA) with detailed specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. The product is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to minimize exposure to atmospheric moisture. For long-term storage, consider desiccants.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Heavy Metals	< 10 ppm
Residual Solvents (GC)	Complies with ICH guidelines
Biological Activity	Suitable for enzyme assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.