Description

5,5'-Difluoro Bapta, Am is a specialized, high-purity fluorinated derivative of the BAPTA chelator family, designed for advanced research applications. This compound matters for its enhanced selectivity and binding affinity for calcium ions, making it a critical tool in fluorescence-based intracellular calcium sensing and imaging. It is primarily needed by researchers and manufacturers in the fields of biochemistry, molecular biology, and diagnostic reagent development, where precise calcium quantification is paramount.

Application

• Fluorescent Calcium Indicator Synthesis: A key precursor for developing next-generation, cell-permeable calcium-sensitive dyes (e.g., fluo-4, fura-2 analogs) with improved photostability and specificity.
• Biochemical Research: Used in the study of calcium signaling pathways, neurotransmitter release, muscle contraction, and other physiological processes dependent on intracellular calcium flux.
• Diagnostic Kit Manufacturing: Incorporated into assay kits for the quantitative measurement of calcium concentrations in clinical and research laboratory settings.
• Neuroscience and Cell Biology: Essential for real-time visualization and measurement of dynamic calcium changes in neurons, cardiomyocytes, and other cell types using fluorescence microscopy.
• High-Throughput Screening (HTS): Serves as a core component in assays designed for drug discovery, particularly for compounds targeting calcium channels or calcium-dependent enzymes.
• Chelator Chemistry Development: Used as a model compound in research focused on modifying chelator properties for specific metal ions.

Basic Information

Product Name	5,5'-Difluoro Bapta, Am
CAS No.	156027-00-8
Molecular Formula	C22H20F2N2O10
Molecular Weight	510.40 g/mol
Synonyms	5,5'-Difluoro-1,2-bis(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid, acetoxymethyl ester; 5,5'-Difluoro BAPTA AM; 5,5'-F2-BAPTA AM; 5,5'-Difluoro BAPTA, acetoxymethyl ester; 5,5'-Bis(fluoranyl)-BAPTA/AM; [1,2-Bis(2-amino-5-fluorophenoxy)ethane-N,N,N',N'-tetraacetic acid tetrakis(acetoxymethyl) ester]; A cell-permeable, fluorinated calcium chelator precursor.
EINECS	Contact for details

Quality Control

Our 5,5'-Difluoro Bapta, Am is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity, suitable for sensitive research applications. Each lot undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry. Certificates of Analysis (COA) detailing all specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.