Description

1-Oleoyl-2-Hexanoyl-Nbd-Glycerol is a specialized fluorescently-labeled diacylglycerol (DAG) analog, a critical tool compound in biochemical and pharmacological research. Its core value lies in the incorporation of the nitrobenzoxadiazole (NBD) fluorophore, enabling real-time, sensitive detection and visualization of lipid-protein interactions and metabolic pathways. This product is essential for researchers and developers in lipidomics, cell signaling studies, and drug discovery, particularly for investigating enzymes like protein kinase C (PKC), phospholipases, and diacylglycerol kinases.

Application

• Biochemical Research: As a fluorescent substrate for studying the kinetics and mechanisms of lipases, phospholipases, and other lipid-metabolizing enzymes.
• Cell Signaling Studies: Probing the role of diacylglycerol (DAG) as a second messenger in cellular pathways, including PKC activation and membrane trafficking.
• Membrane Biophysics: Investigating lipid bilayer properties, membrane fusion, and lipid-protein interactions using fluorescence-based assays.
• Drug Discovery Screening: Serving as a key reagent in high-throughput screens for identifying modulators of lipid-signaling pathways relevant to metabolic and inflammatory diseases.
• Lipidomics and Metabolic Tracing: Utilizing the NBD tag to track the metabolism and intracellular localization of diacylglycerol species.
• Diagnostic Assay Development: Acting as a core component in developing clinical diagnostic kits for enzyme deficiencies related to lipid metabolism.

Basic Information

Product Name	1-Oleoyl-2-Hexanoyl-Nbd-Glycerol
CAS No.	155581-64-9
Molecular Formula	C34H49N3O8
Molecular Weight	627.78 g/mol
Synonyms	1-Oleoyl-2-hexanoyl-sn-glycerol-3-(NBD); NBD-C6/C18:1-DAG; 1-Oleoyl-2-caproyl-sn-glycerol labeled with NBD; Fluorescent diacylglycerol analog; 2-[(7-Nitro-2,1,3-benzoxadiazol-4-yl)amino]-1,3-propanediol dioleate hexanoate derivative; DAG-NBD (C6:0/C18:1); sn-1-Oleoyl-2-hexanoylglycerol-3-NBD
EINECS	Contact for details

Quality Control

Our 1-Oleoyl-2-Hexanoyl-Nbd-Glycerol is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation (NMR, MS). A detailed Certificate of Analysis (COA) is provided with every shipment, documenting the exact specifications and results. We support research compliance with traceable documentation.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen). The product is light-sensitive and should be handled under subdued light. Allow the sealed vial to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange viscous oil or solid
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95% (Area %)
Solubility	Soluble in DMSO, DMF, chloroform, ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.