Description

3'-Deoxythymidine-5'-o-(1-Thiotriphosphate)/2',3'-Dideoxythymidine-5'-o-(1-Thiotriphosphate) Sodium Salt is a high-purity, modified nucleotide analog essential for advanced molecular biology and biochemistry research. This compound is a critical reagent in enzymatic synthesis and sequencing applications, where its sulfur-for-oxygen substitution provides unique biochemical properties. It is primarily utilized by researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic sectors for developing novel therapeutic and analytical methods.

Application

• Enzymatic DNA/RNA Synthesis: Used as a substrate for DNA polymerases in the synthesis of oligonucleotides containing phosphorothioate linkages, which confer nuclease resistance.
• Next-Generation Sequencing (NGS): Serves as a key component in sequencing-by-synthesis (SBS) technologies and other advanced sequencing platforms.
• Antiviral & Anticancer Research: Investigated as a chain-terminating nucleotide analog in the development of nucleoside reverse transcriptase inhibitors (NRTIs) and other therapeutic agents.
• Biochemical Assay Development: Employed in the creation of sensitive assays for enzyme activity, including studies on polymerase fidelity and kinetics.
• Molecular Diagnostics: Used in the production of probes and primers for PCR-based diagnostic tests, enhancing stability and specificity.
• Structural Biology Studies: Acts as a tool for investigating enzyme-substrate interactions and the mechanisms of nucleic acid polymerases through X-ray crystallography and NMR.

Basic Information

Item	Detail
Product Name	3'-Deoxythymidine-5'-o-(1-Thiotriphosphate)/2',3'-Dideoxythymidine-5'-o-(1-Thiotriphosphate) Sodium Salt
CAS No.	154771-48-9
Molecular Formula	C10H14N2NaO13P3S
Molecular Weight	526.17 g/mol
Synonyms	2',3'-Dideoxythymidine 5'-(1-Thiotriphosphate), Sodium Salt; ddTTPαS, Sodium Salt; 3'-Deoxythymidine-5'-(α-thiotriphosphate); Thio-dideoxythymidine triphosphate; Sp-isomer of ddTTPαS; Nucleotide analog; Phosphorothioate nucleotide; Chain-terminating nucleotide
EINECS	Contact for details

Quality Control

Every batch of our 3'-Deoxythymidine-5'-o-(1-Thiotriphosphate)/2',3'-Dideoxythymidine-5'-o-(1-Thiotriphosphate) Sodium Salt is manufactured under strict quality management systems. We employ advanced analytical techniques, including HPLC, NMR, and mass spectrometry, to ensure high purity and batch-to-batch consistency suitable for sensitive research applications. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). For long-term storage, keep desiccated under an inert atmosphere. Allow the product to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
pH (1% Solution)	6.0 - 8.0
Endotoxin	< 10 EU/mg
Biological Activity	Suitable for enzymatic incorporation

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.