Description

Nicotine n-β-D-Glucuronide is a major phase II metabolite of nicotine, formed via glucuronidation. This compound is of critical importance for analytical and pharmacological research into nicotine metabolism, exposure, and pharmacokinetics. It serves as a primary reference standard for laboratories in the pharmaceutical, clinical diagnostics, and tobacco research sectors requiring precise quantification of nicotine metabolites in biological matrices.

Application

• Biomarker Reference Standard: Primary use as a certified reference material (CRM) for the quantification of nicotine and its metabolites in human urine, plasma, and serum in clinical and forensic toxicology.
• Pharmacokinetic & Metabolic Studies: Essential for in vitro and in vivo research investigating the absorption, distribution, metabolism, and excretion (ADME) profile of nicotine and related alkaloids.
• Diagnostic Assay Development & Calibration: Critical component for the development, validation, and calibration of immunoassays, LC-MS/MS, and HPLC-UV methods used in diagnostic kits and laboratory testing.
• Tobacco Exposure Research: Used in studies assessing smoking status, nicotine replacement therapy (NRT) efficacy, and exposure to secondhand smoke.
• Pharmaceutical Impurity Profiling: Acts as a specified impurity reference standard in the quality control of nicotine-based pharmaceutical products like gums, patches, and lozenges.
• Regulatory Compliance & Proficiency Testing: Employed in laboratories to ensure compliance with methods mandated by health agencies and in inter-laboratory proficiency testing schemes.

Basic Information

Product Name	Nicotine n-β-D-Glucuronide
CAS No.	153536-53-9
Molecular Formula	C16H22N2O7
Molecular Weight	354.36 g/mol
Synonyms	Nicotine N-Glucuronide; Nicotine 1'-β-D-Glucuronide; (S)-Nicotine N-β-D-Glucuronide; Nicotine N-β-D-Glucopyranosiduronic Acid; trans-Nicotine N-β-D-Glucuronide; Nicotine N(1)-β-D-Glucuronide; Nicotine Glucuronide; Major Nicotine Metabolite
EINECS	Contact for details

Quality Control

Our Nicotine n-β-D-Glucuronide is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation (NMR, MS), to ensure it meets the high standards required for reference materials. A detailed Certificate of Analysis (COA) providing batch-specific data is supplied with every order.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry, cool place. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥95% (Area %)
Specific Rotation	Contact for details
Water Content (KF)	≤5.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.