Description

o-Nitrophenyl-n-Acetyl-β-D-Galactosaminide is a specialized chromogenic substrate used in enzymatic assays. This compound is critical for the detection and quantification of β-galactosidase activity, a key enzyme in molecular biology and diagnostic research. It is primarily required by biochemical researchers, diagnostic kit manufacturers, and pharmaceutical quality control laboratories for applications in gene expression studies, reporter assays, and enzyme-linked diagnostics.

Application

• Enzymatic Substrate for β-Galactosidase: Used as a chromogenic reagent in colorimetric assays to measure β-galactosidase enzyme activity in research and diagnostics.
• Molecular Biology Research: Essential in reporter gene assays (e.g., lacZ gene expression) for studying gene regulation and promoter activity.
• Diagnostic Kit Manufacturing: Incorporated into commercial test kits for the detection of bacterial enzymes, such as in microbiology identification systems.
• Cell Biology & Histochemistry: Employed in staining protocols to visualize β-galactosidase activity in tissues and cultured cells.
• Pharmaceutical QC Testing: Used in quality control procedures for products involving recombinant proteins or gene therapy vectors that utilize the lacZ system.
• Biochemical Reagent: Serves as a high-purity reference standard and critical raw material for biochemical and analytical laboratories.

Basic Information

Product Name	o-Nitrophenyl-n-Acetyl-β-D-Galactosaminide
CAS No.	152957-34-1
Molecular Formula	C14H18N2O8
Molecular Weight	342.30 g/mol
Synonyms	o-Nitrophenyl N-Acetyl-β-D-galactosaminide; 2-Nitrophenyl 2-acetamido-2-deoxy-β-D-galactopyranoside; ONP-NAG; ONP-GalNAc; 2-Nitrophenyl N-acetyl-β-D-galactosaminide; N-Acetyl-β-D-galactosaminide, 2-nitrophenyl; β-D-GalNAc-ONP; o-Nitrophenyl β-D-GalNAc
EINECS	Contact for details

Quality Control

Our o-Nitrophenyl-n-Acetyl-β-D-Galactosaminide is manufactured under strict quality systems to ensure high purity and batch-to-batch consistency suitable for sensitive research and diagnostic applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard enzymatic assays. We adhere to rigorous in-house specifications to meet the demands of the life science industry.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation. For long-term storage, consider desiccants.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Specific Rotation	Contact for details
Enzymatic Activity	Conforms to reference standard
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.