Description

Valyl-Leucyl-Lysyl-7-Amino-4-Methylcoumarin is a specialized fluorogenic peptide substrate designed for high-sensitivity enzymatic assays. This compound is critical for researchers and quality control laboratories requiring precise detection and quantification of specific protease activity. It is primarily utilized in the life sciences, pharmaceutical development, and diagnostic reagent manufacturing sectors for studying enzyme kinetics and screening inhibitors.

Application

• Protease Activity Assays: As a fluorogenic substrate for the continuous, real-time monitoring of trypsin, plasmin, and related serine proteases.
• High-Throughput Screening (HTS): Used in drug discovery platforms to identify and characterize novel protease inhibitors for therapeutic targets.
• Biochemical Research: A key tool for investigating protease function, specificity, and catalytic mechanisms in vitro.
• Diagnostic Reagent Formulation: Incorporated into kits for the clinical measurement of specific enzyme levels relevant to disease states.
• Quality Control in Biologics: Employed to assess the presence and activity of residual proteases in biopharmaceutical production processes.
• Cell Biology Studies: Used to detect and localize protease activity within cellular and tissue samples.

Basic Information

Product Name	Valyl-Leucyl-Lysyl-7-Amino-4-Methylcoumarin
CAS No.	152881-17-9
Molecular Formula	C33H51N7O7
Molecular Weight	657.81 g/mol
Synonyms	Val-Leu-Lys-AMC; VLK-AMC; H-Val-Leu-Lys-AMC; 7-Amino-4-methylcoumarin conjugate of Val-Leu-Lys; Fluorogenic substrate VLK-AMC; Trypsin substrate VLK-AMC; Boc-Val-Leu-Lys-AMC (protected form); Valylleucyllysyl-7-amino-4-methylcoumarin
EINECS	Contact for details

Quality Control

Our Valyl-Leucyl-Lysyl-7-Amino-4-Methylcoumarin is manufactured under strict quality systems to ensure batch-to-batch consistency and reliability for critical research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard enzymatic assays. We support compliance with relevant GMP and ISO guidelines for raw materials used in pharmaceutical and diagnostic development.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Functional Activity	Conforms to reference standard in trypsin assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.