Description

92 Kda Gelatinase, Proenzyme is a high-purity, latent form of the 92 kDa type IV collagenase, a key metalloproteinase involved in extracellular matrix remodeling. This proenzyme form is critical for research requiring controlled activation, offering superior stability and reduced nonspecific proteolytic activity prior to use. It is an essential biochemical tool for researchers in oncology, cell biology, and tissue engineering, facilitating studies on tumor metastasis, angiogenesis, and cellular invasion mechanisms.

Application

• Cancer Research & Metastasis Studies: Investigation of tumor cell invasion and migration through basement membrane models, particularly for studying MMP-9 activation pathways.
• Angiogenesis Assays: Used in *in vitro* models to study the role of gelatinases in blood vessel formation and endothelial cell behavior.
• Extracellular Matrix (ECM) Remodeling Research: A key reagent for analyzing the degradation of type IV collagen and gelatin in physiological and pathological processes.
• Enzyme Activation & Zymogen Studies: Ideal for biochemical studies on the mechanisms of proenzyme activation by other proteases or chemical agents.
• Drug Discovery & Screening: Serves as a target for developing and testing novel MMP-9 inhibitors for therapeutic applications in cancer and inflammatory diseases.
• Cell Culture & 3D Model Systems: Employed to modify hydrogel matrices or create defined microenvironments for advanced cell culture applications.

Basic Information

Product Name	92 Kda Gelatinase, Proenzyme
CAS No.	152787-66-1
Molecular Formula	Contact for details
Molecular Weight	~92,000 Da (Proenzyme form)
Synonyms	92 kDa Gelatinase Proenzyme
Pro-MMP-9
Matrix Metalloproteinase-9 Proenzyme
92 kDa Type IV Collagenase Proenzyme
Gelatinase B Pro-form
MMP-9 Zymogen
92 kDa Gelatinase Latent Form
EINECS	Contact for details

Quality Control

Our 92 Kda Gelatinase, Proenzyme is produced under stringent conditions to ensure batch-to-batch consistency and high biological activity. Each lot undergoes rigorous quality testing, including purity assessment by SDS-PAGE and activity profiling via zymography, to ensure compliance with research-grade standards. A comprehensive Certificate of Analysis (COA) detailing purity, concentration, and activity data is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized material at -20°C or below. Upon reconstitution, aliquot and store at -70°C to -80°C to minimize freeze-thaw cycles and activity loss. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (SDS-PAGE)	Single major band at ~92 kDa under non-reducing conditions
Purity (SDS-PAGE)	≥ 90%
Activity (Zymography)	Latent proenzyme; activates to show gelatinolytic activity
Protein Content (Bradford)	≥ 90% of stated weight
Endotoxin Level (LAL)	< 1.0 EU/µg
Solubility	Soluble in aqueous buffers or culture medium

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.