Description

Adenosine-2,8-3H 5'-Triphosphate Tetraammonium Salt is a high-specific-activity, tritium-labeled derivative of adenosine triphosphate (ATP), a fundamental molecule for cellular energy transfer. This radiolabeled compound is essential for sensitive biochemical research, enabling the precise tracing and quantification of ATP-dependent enzymatic processes. It is a critical reagent for researchers in molecular biology, enzymology, and drug discovery, particularly in studies involving kinases, ATPases, and other nucleotide-binding proteins.

Application

• Enzyme Kinetics & Mechanism Studies: Used as a radiolabeled substrate to investigate the kinetics, binding affinity, and catalytic mechanisms of ATP-utilizing enzymes like kinases, ATPases, and synthetases.
• High-Throughput Screening (HTS): Employed in scintillation proximity assays (SPA) and other radiometric assays to screen for inhibitors or activators of target enzymes in pharmaceutical discovery pipelines.
• Metabolic Pathway Tracing: Facilitates the study of energy metabolism, phosphorylation cascades, and nucleotide turnover in cell lysates or purified systems.
• Receptor Binding Assays: Serves as a tool in studies of purinergic receptors (P2 receptors) that recognize ATP as a signaling molecule.
• Biomolecular Interaction Analysis: Used in techniques like filter-binding assays to study protein-nucleotide interactions with high sensitivity.
• Quality Control for Diagnostic Kits: Acts as a reference standard in the development and validation of diagnostic assays that measure ATP-related activities.

Basic Information

Product Name	Adenosine-2,8-3H 5'-Triphosphate Tetraammonium Salt
CAS No.	152585-45-0
Molecular Formula	C10H11N5O13P3 · x(C3H) · 4NH3
Molecular Weight	~551.1 (unlabeled ATP tetraammonium salt base) + labeled mass
Synonyms	[3H]ATP; Tritiated ATP; Adenosine 5'-(tetrahydrogen triphosphate), 2,8-3H, tetraammonium salt; 2,8-3H-Adenosine-5'-triphosphate tetraammonium salt; [2,8-3H]ATP (NH4)4 salt; ATP, [2,8-3H]-; Radiolabeled ATP tetraammonium salt
EINECS	Contact for details

Quality Control

Every batch of our radiolabeled nucleotides is produced and tested to meet the exacting standards required for life science research. Rigorous analytical controls, including HPLC and radiometric purity analysis, ensure high specific activity and chemical stability. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing specific activity (Ci/mmol or GBq/mmol), radiochemical purity, chemical purity, and solvent composition. Our quality system supports traceability and consistency for critical research applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at ≤ -20°C or as specified on the label. The product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to prevent condensation. Store in accordance with local regulations for radioactive materials.

Specification

Item	Specification
Appearance	Clear, colorless to slightly yellow solution
Identification (HPLC/Radio-TLC)	Conforms to reference standard
Specific Activity	20-50 Ci/mmol (740-1850 GBq/mmol) [Typical]
Radiochemical Purity (HPLC)	≥ 95%
Chemical Purity (HPLC, UV)	≥ 90%
Concentration	1 mCi/mL (37 MBq/mL) [Typical, in aqueous ethanol]
Solvent System	Ethanol:Water (e.g., 7:3 v/v)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.