Description

3-((4-Methoxyspiro(1,2-Dioxetane-3,2'-Tricyclo(3.3.1.1(3,7))Decan)-4-Yl))Phenyl Myo-Inositol-1-o-Hydrogen Phosphate is a specialized chemiluminescent derivative of myo-inositol phosphate, designed for high-sensitivity detection and analysis. This compound matters for its ability to act as a highly efficient substrate in enzymatic assays, particularly for phosphatases, generating a strong, measurable light signal. It is primarily needed by researchers and manufacturers in the pharmaceutical, diagnostic, and life science research sectors for developing advanced bioanalytical kits and assays.

Application

• Chemiluminescent Substrate for Phosphatase Assays: Used as a key component in ultra-sensitive detection kits for alkaline phosphatase (ALP) and other phosphatases in ELISA and Western blotting.
• Diagnostic Kit Manufacturing: Integrated into clinical diagnostic kits for the detection of biomarkers, hormones, and infectious agents.
• Life Science Research: Enables sensitive detection of enzymatic activity in cell signaling pathway studies, particularly those involving inositol phosphate metabolism.
• High-Throughput Screening (HTS): Employed in drug discovery platforms to identify enzyme inhibitors or activators due to its robust signal-to-noise ratio.
• Bioluminescence Resonance Energy Transfer (BRET): Serves as a core component in advanced BRET-based probes for real-time monitoring of biological interactions.
• DNA/RNA Probe Detection: Utilized in nucleic acid hybridization assays where enzyme-labeled probes require highly sensitive chemiluminescent detection.

Basic Information

Item	Details
Product Name	3-((4-Methoxyspiro(1,2-Dioxetane-3,2'-Tricyclo(3.3.1.1(3,7))Decan)-4-Yl))Phenyl Myo-Inositol-1-o-Hydrogen Phosphate
CAS No.	152305-53-8
Molecular Formula	C25H31O11P
Molecular Weight	538.49 g/mol
Synonyms	3-(4-Methoxyspiro[1,2-dioxetane-3,2'-tricyclo[3.3.1.13,7]decan]-4-yl)phenyl myo-inositol 1-phosphate; AMPPD derivative; Chemiluminescent Inositol Phosphate; Dioxetane Phosphate Substrate; Lumigen PS-3; 1D-myo-Inositol 1-(3-(4-methoxyspiro[1,2-dioxetane-3,2'-tricyclo[3.3.1.13,7]decan]-4-yl)phenyl) phosphate; Alkaline Phosphatase Substrate (Chemiluminescent); CSPD® analog
EINECS	Contact for details

Quality Control

Our production of this high-purity chemiluminescent substrate adheres to stringent quality protocols. Every batch undergoes comprehensive analysis, including HPLC for purity and identity confirmation, to ensure performance consistency and reliability in sensitive assay systems. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are provided with each shipment. We support compliance with relevant GMP and ISO 13485 standards for diagnostic manufacturers.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is light-sensitive (store away from light), easily oxidized (store under inert atmosphere), and hygroscopic (moisture-sensitive). For long-term stability, store under an argon or nitrogen atmosphere. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Chemiluminescent Activity	≥ 95% of reference standard activity
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.