Description

Benzyloxycarbonylleucyl-Leucyl-Leucyl-4-Methyl-Coumaryl-7-Amide is a specialized fluorogenic peptide substrate designed for high-sensitivity enzymatic assays. This compound is critically important for researchers and quality control laboratories requiring precise measurement of proteolytic activity, particularly from enzymes like the proteasome. It is primarily needed by the pharmaceutical, biotechnology, and academic research sectors for drug discovery, biochemical pathway analysis, and enzyme characterization studies.

Application

• Proteasome Activity Assays: A key fluorogenic substrate for measuring chymotrypsin-like activity of the 20S and 26S proteasome complexes in cell lysates and purified systems.
• Drug Discovery & Screening: Used in high-throughput screening (HTS) platforms to identify and characterize novel proteasome inhibitors for cancer and inflammatory disease therapeutics.
• Biochemical Research: Employed in kinetic studies to determine enzyme inhibition constants (Ki, IC50) and elucidate mechanisms of proteasome function and regulation.
• Diagnostic Development: Serves as a critical reagent in developing diagnostic tests for diseases where proteasome activity is a biomarker.
• Quality Control in Biologics: Used in QC labs for monitoring the activity and consistency of enzyme-based therapeutic products.

Basic Information

Product Name	Benzyloxycarbonylleucyl-Leucyl-Leucyl-4-Methyl-Coumaryl-7-Amide
CAS No.	152015-61-7
Molecular Formula	C43H60N6O9
Molecular Weight	805.0 g/mol
Synonyms	Z-Leu-Leu-Leu-AMC; Z-LLL-AMC; Z-Leu-Leu-Leu-7-amido-4-methylcoumarin; Cbz-Leu-Leu-Leu-AMC; Suc-LLVY-AMC (related activity); Proteasome Substrate III; Chymotrypsin-like Proteasome Substrate; Fluorogenic Proteasome Substrate; Calpain Inhibitor I (related); Ac-Leu-Leu-Nle-CHO (related inhibitor).
EINECS	Contact for details

Quality Control

Our Benzyloxycarbonylleucyl-Leucyl-Leucyl-4-Methyl-Coumaryl-7-Amide is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to meet the exacting standards of research and pharmaceutical applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and functional performance data is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (Mass Spec)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Assay (Functional)	≥ 98.0% (enzymatic)
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.