Description

2,3-Bis(4-Methoxyphenyl)-5-(4-Cyanophenyl)Tetrazolium Chloride is a specialized tetrazolium salt derivative, primarily recognized for its role as a vital biochemical reagent. This compound matters for its ability to serve as a sensitive redox indicator in cell viability and cytotoxicity assays, providing critical data for research and development. It is needed by professionals in the pharmaceutical, biotechnology, and life science research sectors for applications in drug discovery, metabolic studies, and diagnostic development.

Application

• Cell Viability and Proliferation Assays: Used as a key substrate in colorimetric assays (e.g., MTT-like assays) to measure mitochondrial activity in live cells.
• Cytotoxicity Screening: Employed in high-throughput screening platforms to evaluate the toxic effects of novel drug candidates or chemical compounds on cultured cells.
• Microbiological Research: Applied in studies of microbial metabolism and for assessing the efficacy of antimicrobial agents.
• Biochemical Research: Functions as an electron acceptor in enzymatic assays to study dehydrogenase activity and other redox processes.
• Diagnostic Kit Manufacturing: Incorporated as a core component in the formulation of diagnostic kits for clinical and research laboratories.
• Apoptosis and Cell Death Studies: Utilized to distinguish between apoptotic, necrotic, and healthy cell populations.

Basic Information

Product Name	2,3-Bis(4-Methoxyphenyl)-5-(4-Cyanophenyl)Tetrazolium Chloride
CAS No.	151390-91-9
Molecular Formula	C28H21ClN6O2
Molecular Weight	509.96 g/mol
Synonyms	5-(4-Cyanophenyl)-2,3-bis(4-methoxyphenyl)tetrazolium chloride; Tetrazolium Chloride, 2,3-Bis(4-methoxyphenyl)-5-(4-cyanophenyl)-; WST-8 (related tetrazolium salt class); CCK-8 Assay Reagent (related application); Cell Counting Kit-8 component analog; Water-soluble tetrazolium salt derivative; Redox indicator for cell assays; MTT analog
EINECS	Contact for details

Quality Control

Our 2,3-Bis(4-Methoxyphenyl)-5-(4-Cyanophenyl)Tetrazolium Chloride is produced under strict quality management systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot undergoes rigorous analytical testing, including HPLC for purity verification and spectroscopic identification. Certificates of Analysis (COA) detailing purity, identity, and performance data are available upon request to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept tightly sealed in a desiccated environment to prevent degradation. For long-term stability, consider storage under an inert atmosphere.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.5%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Assay (Functional)	Performance tested in standard cell viability assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.