Description

Thymidin-3'-Yl-4-Thiothymidin-5'-Yl Methylphosphonate is a chemically modified nucleoside analog, specifically a dinucleotide methylphosphonate thioate. This compound is of significant value in advanced biochemical research and pharmaceutical development due to its role as a building block for antisense oligonucleotides and other therapeutic nucleic acid analogs. It is primarily needed by research institutions, biotechnology companies, and pharmaceutical developers working on gene silencing, diagnostic probes, and novel therapeutic modalities. The precise structure allows for enhanced nuclease resistance and target binding affinity in oligonucleotide synthesis.

Application

• Antisense Oligonucleotide (ASO) Synthesis: A critical phosphorothioate-modified building block for creating therapeutic oligonucleotides that modulate gene expression.
• Research & Diagnostic Probe Development: Used in the construction of labeled or unlabeled probes for studying gene function, viral detection, and in situ hybridization.
• siRNA and miRNA Mimic/Antagonist Synthesis: Serves as a modified unit to enhance the stability and pharmacokinetic properties of RNA-based therapeutics.
• Pharmaceutical Intermediate: An essential raw material in the production pipeline for novel nucleic acid-based drugs targeting cancers, genetic disorders, and viral infections.
• Bioconjugation and Labeling Studies: The reactive sites allow for further functionalization with fluorophores, quenchers, or targeting ligands for advanced biomedical applications.
• Structural Biology and Mechanistic Studies: Used to investigate enzyme mechanisms (e.g., polymerases, nucleases) and the structure-function relationships of nucleic acids.

Basic Information

Product Name	Thymidin-3'-Yl-4-Thiothymidin-5'-Yl Methylphosphonate
CAS No.	151165-76-3
Molecular Formula	C21H28N4O12P2S
Molecular Weight	622.48 g/mol
Synonyms	4-Thiothymidine Methylphosphonate Dimer; d(TpsT) Methylphosphonate; 3'-Thymidinyl-5'-thymidinyl Methylphosphonate 4-Thio Derivative; Thymidine-3'→5'-4-Thiothymidine Methylphosphonate; Anti-sense Oligonucleotide Intermediate; 4-Thio-dT Methylphosphonate Dimer; 2'-Deoxythymidin-3'-yl 5'-(4-thio-2'-deoxythymidin-5'-yl) methylphosphonate
EINECS	Contact for details

Quality Control

Our production of Thymidin-3'-Yl-4-Thiothymidin-5'-Yl Methylphosphonate adheres to stringent quality protocols suitable for research and pharmaceutical intermediate applications. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and MS for identity verification. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are provided with every shipment to ensure traceability and compliance with your research or cGMP starting material requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions or a dry inert atmosphere to prevent degradation. Allow the sealed container to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.