Description

n-(2-(3-(Trifluoromethyl)Diazirin-3-Yl)Phenyl)-4-Maleimidobutyramide is a specialized heterobifunctional crosslinking reagent designed for advanced photochemical and biochemical applications. Its core value lies in the unique combination of a photoactivatable trifluoromethyl diazirine group and a maleimide moiety, enabling precise, light-induced covalent bonding to target molecules under mild conditions. This compound is essential for researchers and developers in proteomics, structural biology, and materials science who require high-purity, reliable reagents for photoaffinity labeling, surface functionalization, and the development of novel biomaterials.

Application

• Photoaffinity Labeling (PAL): A critical tool for mapping protein-protein interactions, identifying binding sites, and studying ligand-receptor dynamics by forming irreversible crosslinks upon UV light activation.
• Bioconjugation and Probe Synthesis: Used to create stable conjugates between biomolecules (like proteins, peptides, or oligonucleotides) and reporter molecules (e.g., fluorophores, biotin) via the maleimide-thiol chemistry.
• Surface Immobilization and Biointerface Engineering: Functionalizes gold, glass, or polymer surfaces for biosensor development, microarray fabrication, and creating bioactive coatings.
• Polymer and Materials Modification: Serves as a crosslinker to introduce photoreactive sites into hydrogels, nanoparticles, or other polymeric materials for controlled property tuning.
• Chemical Biology and Drug Discovery: Facilitates target identification and validation for novel therapeutic compounds through covalent capture strategies.
• Structural Biology Studies: Aids in the stabilization of transient complexes for analysis by techniques such as cryo-electron microscopy (cryo-EM) or X-ray crystallography.

Basic Information

Item	Details
Product Name	n-(2-(3-(Trifluoromethyl)Diazirin-3-Yl)Phenyl)-4-Maleimidobutyramide
CAS No.	151077-62-2
Molecular Formula	C20H15F3N4O3
Molecular Weight	416.36 g/mol
Synonyms	4-Maleimidobutyric acid N-(2-(3-(trifluoromethyl)-3H-diazirin-3-yl)phenyl)amide; 3-(Trifluoromethyl)-3-(m-isothiocyanatophenyl)diazirine-4-maleimidobutyramide derivative; TFD-Maleimide; Photoactivatable Maleimide Crosslinker; Heterobifunctional Diazirine-Maleimide Crosslinker; PAL Maleimide Reagent; (2-(3-(Trifluoromethyl)diazirin-3-yl)phenyl)-4-maleimidobutyramide
EINECS	Contact for details

Quality Control

Our n-(2-(3-(Trifluoromethyl)Diazirin-3-Yl)Phenyl)-4-Maleimidobutyramide is manufactured under strict quality protocols to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical characterization, including HPLC for purity verification, NMR for structural confirmation, and mass spectrometry for identity. Certificates of Analysis (COA) detailing all specifications are provided with every shipment. We adhere to cGMP principles where applicable to support our clients in research and development phases.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen). The product is strictly light-sensitive and must be handled under subdued light or in amber glass vials to prevent premature activation of the diazirine group. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.