Description

(2-(4-Iodophenyl)-3-(4-Nitrophenyl)-5-(2,4-Disulfophenyl)-2H-Tetrazolium Sodium Salt is a specialized tetrazolium salt derivative, a critical reagent in biochemical and cell-based assays. Its core value lies in its role as a chromogenic substrate for dehydrogenase enzymes, producing a highly colored formazan product for sensitive detection and quantification. This compound is essential for researchers and quality control professionals in the pharmaceutical, biotechnology, and life sciences industries who require reliable, high-purity reagents for viability, cytotoxicity, and metabolic activity testing.

Application

• Cell Viability and Proliferation Assays: Used as a key substrate in MTT and related assays to measure mitochondrial activity in live cells.
• Cytotoxicity Screening: Employed in high-throughput screening (HTS) platforms to evaluate the toxic effects of drug candidates or chemical compounds on cell cultures.
• Microbial Assays: Applied in microbiology for assessing bacterial or fungal metabolic activity and susceptibility testing.
• Biochemical Enzyme Assays: Serves as an electron acceptor in dehydrogenase enzyme assays for research and diagnostic purposes.
• Apoptosis Detection: Utilized in assays designed to detect programmed cell death and related metabolic changes.
• Drug Discovery & Development: A critical tool in preclinical research for evaluating the efficacy and safety profiles of new therapeutic agents.
• Quality Control in Biomanufacturing: Used to monitor cell culture health and consistency in biopharmaceutical production processes.

Basic Information

Product Name	(2-(4-Iodophenyl)-3-(4-Nitrophenyl)-5-(2,4-Disulfophenyl)-2H-Tetrazolium Sodium Salt
CAS No.	150849-52-8
Molecular Formula	C19H11IN5Na2O10S2
Molecular Weight	779.33 g/mol
Synonyms	WST-3; 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, disodium salt; Iodonitrotetrazolium Violet, disulfonated form; INT-Violet, disodium salt; Water-Soluble Tetrazolium Salt 3; 4-[3-(4-Iodophenyl)-2-(4-nitrophenyl)-2H-tetrazol-3-ium-5-yl]benzene-1,3-disulfonate, sodium salt
EINECS	Contact for details

Quality Control

Our (2-(4-Iodophenyl)-3-(4-Nitrophenyl)-5-(2,4-Disulfophenyl)-2H-Tetrazolium Sodium Salt is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, spectrophotometry for performance validation, and residual solvent analysis, to ensure it meets the high standards required for sensitive research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing identity, purity, assay, and related substances is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry, cool place. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store desiccated under inert atmosphere. Allow the sealed container to reach room temperature before opening to prevent condensation and moisture ingress.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.5%
Heavy Metals	≤ 20 ppm
Assay (Spectrophotometric)	95.0 - 105.0%
Solubility (in Water)	Clear, yellow solution at 10 mg/mL
Biological Performance	Passes cell-based assay validation

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.