Description

n-Succinimidyl 7-Methoxycoumarin-3-Carboxylate is a highly reactive, amine-reactive fluorescent labeling reagent. This compound is critical for researchers and manufacturers who require a stable, fluorescent tag for biomolecules such as proteins, peptides, and antibodies. It is primarily used in the life sciences, pharmaceutical development, and diagnostic industries for applications in fluorescence microscopy, immunoassays, and biochemical analysis.

Application

• Fluorescent Labeling of Biomolecules: Primary application for covalently tagging primary amines on proteins, peptides, and antibodies for detection and quantification.
• Proteomics Research: Used in gel electrophoresis and Western blotting as a pre-staining or post-staining fluorescent marker.
• Diagnostic Assay Development: Serves as a key component in developing fluorescent immunoassays (FIAs) and enzyme-linked immunosorbent assays (ELISAs).
• Cell Biology & Microscopy: Enables the fluorescent labeling of cellular components for real-time imaging and tracking in live or fixed cells.
• High-Throughput Screening (HTS): Utilized in drug discovery platforms to label targets for fluorescence-based activity and binding assays.
• Bioconjugation & Chemical Biology: A versatile building block for creating custom fluorescent probes and antibody-drug conjugates (ADCs).
• Quality Control in Biomanufacturing: Used to label and trace proteins during purification and formulation processes.

Basic Information

Product Name	n-Succinimidyl 7-Methoxycoumarin-3-Carboxylate
CAS No.	150321-92-9
Molecular Formula	C15H11NO7
Molecular Weight	317.25 g/mol
Synonyms	7-Methoxycoumarin-3-carboxylic acid N-succinimidyl ester; 7-Methoxycoumarin-3-carboxylic acid, N-hydroxysuccinimide ester; 3-Carboxy-7-methoxycoumarin N-succinimidyl ester; CMCM-OSu; 7-Methoxy-3-carboxycoumarin NHS ester; Succinimidyl 7-methoxy-3-coumarincarboxylate; 7-Methoxycoumarin-3-carbonyloxysuccinimide
EINECS	Contact for details

Quality Control

Our n-Succinimidyl 7-Methoxycoumarin-3-Carboxylate is manufactured under strict quality protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research and development applications. Each lot is characterized by HPLC, NMR, and mass spectrometry to confirm identity and purity. Certificates of Analysis (COA) detailing all quality parameters are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Off-white to pale yellow crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.