Description

6-Carboxyfluorescein 3’,6’-Diacetate n-Succinimidyl Ester is a reactive, amine-reactive fluorescent labeling reagent widely used in biochemistry and molecular biology. This compound is critical for creating stable, fluorescently tagged biomolecules, enabling sensitive detection and analysis in complex systems. It is primarily needed by researchers and manufacturers in the life sciences, pharmaceutical development, and diagnostic assay industries for applications such as protein conjugation, nucleic acid labeling, and cell tracing.

Application

• Fluorescent Labeling of Proteins and Antibodies: Conjugation to primary amines (e.g., lysine residues) for use in fluorescence microscopy, flow cytometry (FACS), and immunoassays (ELISA).
• Nucleic Acid Probe Synthesis: Labeling of oligonucleotides for fluorescence in situ hybridization (FISH) and real-time PCR applications.
• Cell Tracing and Viability Assays: Intracellular labeling of peptides or other molecules for tracking cell migration, proliferation, and apoptosis.
• Bioconjugation and Linker Chemistry: Serving as a key intermediate for synthesizing more complex fluorescent dyes and sensor molecules.
• Diagnostic Kit Manufacturing: Production of fluorescent detection components for clinical diagnostic tests and point-of-care devices.
• High-Throughput Screening (HTS): Development of fluorescent probes for drug discovery and biochemical pathway analysis.

Basic Information

Product Name	6-Carboxyfluorescein 3’,6’-Diacetate n-Succinimidyl Ester
CAS No.	150206-15-8
Molecular Formula	C29H21NO11
Molecular Weight	559.48 g/mol
Synonyms	6-CFSE; 6-Carboxyfluorescein Diacetate Succinimidyl Ester; 6-Carboxyfluorescein 3',6'-Diacetate, Succinimidyl Ester; 6-FAM, SE, Diacetate; 6-Carboxyfluorescein Diacetate, N-Succinimidyl Ester; 6-CFDA, SE; 5(6)-Carboxyfluorescein Diacetate N-Succinimidyl Ester; CFDA, SE (6-isomer)
EINECS	Contact for details

Quality Control

Our 6-Carboxyfluorescein 3’,6’-Diacetate n-Succinimidyl Ester is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets the high standards required for sensitive research and diagnostic applications. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC)	Conforms
Identification (IR)	Conforms
Purity (HPLC)	≥ 95.0%
Single Largest Impurity (HPLC)	≤ 2.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.