Description

4-Nitrophenyl o- (2-Acetamido-2-Deoxyglucopyranosyl)-(1-2)-o-(4,6-Di-o-Methylmannopyranosyl)-(1-6)-Glucopyranoside is a specialized synthetic trisaccharide derivative, featuring a 4-nitrophenyl aglycone. This compound is of significant value as a chromogenic substrate for enzymatic studies, particularly in the characterization of glycosyl hydrolases and transferases. It is primarily utilized by researchers and manufacturers in the biochemical, pharmaceutical, and diagnostic sectors for high-precision enzyme activity assays and inhibitor screening.

Application

• Enzyme Substrate for Glycosidases: Acts as a chromogenic substrate for the kinetic analysis and characterization of specific α- and β-glycosidases, including mannosidases and glucosidases.
• Biochemical Research Reagent: Used in academic and industrial research laboratories to study enzyme mechanisms, inhibition, and substrate specificity.
• Pharmaceutical Development: Employed in high-throughput screening (HTS) assays to identify and evaluate potential glycosidase inhibitors for therapeutic applications.
• Diagnostic Kit Component: Serves as a key reagent in the development of diagnostic assays for enzyme-linked metabolic disorders.
• Carbohydrate Chemistry Intermediate: Used as a building block or reference standard in the synthesis of more complex oligosaccharides or glycoconjugates.
• Quality Control in Biomanufacturing: Applied in the QC testing of enzyme preparations to ensure batch-to-batch consistency and activity.

Basic Information

Item	Detail
Product Name	4-Nitrophenyl o- (2-Acetamido-2-Deoxyglucopyranosyl)-(1-2)-o-(4,6-Di-o-Methylmannopyranosyl)-(1-6)-Glucopyranoside
CAS No.	150161-10-7
Molecular Formula	C33H48N2O22
Molecular Weight	824.74 g/mol
Synonyms	p-Nitrophenyl 2-acetamido-2-deoxy-4-O-(4,6-di-O-methyl-α-D-mannopyranosyl)-3-O-α-D-glucopyranosyl-α-D-glucopyranoside; 4-Nitrophenyl 2-acetamido-2-deoxy-4-O-(4,6-di-O-methyl-α-D-mannopyranosyl)-3-O-α-D-glucopyranosyl-α-D-glucopyranoside; Trisaccharide substrate for α-mannosidase; Chromogenic glycosidase substrate; pNP-GlcNAc-Man(Me)2-Glc; 4-NP-α-D-GlcNAc-(1→2)-α-D-Man(4,6-di-OMe)-(1→6)-α-D-Glc
EINECS	Contact for details

Quality Control

Our production of this high-purity biochemical adheres to stringent quality protocols. Each batch is characterized using advanced analytical techniques including HPLC, NMR, and Mass Spectrometry to confirm identity and purity. A comprehensive Certificate of Analysis (COA) is provided, detailing assay purity, impurity profiles, and physical data. We support research and cGMP-grade manufacturing with documentation tailored to meet your specific quality system requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term stability, consider storage under inert atmosphere or desiccant.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Residual Solvents (GC)	Meets ICH guidelines
Specific Rotation	Contact for details
Biological Activity	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.