Description

5'-O-(4,4-Dimethoxytrityl)-2'-O-[(tert-Butyl)dimethylsilyl]-N-[[4-(tert-Butyl)phenoxy]acetyl]cytidine-3'-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite is a highly specialized, protected phosphoramidite monomer essential for the solid-phase synthesis of oligonucleotides. This compound matters because it enables the precise and efficient incorporation of cytidine analogs into synthetic DNA and RNA strands, a critical process for research and therapeutic development. It is primarily needed by biotechnology and pharmaceutical companies engaged in antisense therapy, siRNA development, diagnostic probe manufacturing, and other advanced nucleic acid applications.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key building block (monomer) in automated DNA/RNA synthesizers for the production of custom sequences.
• Therapeutic Oligonucleotide Manufacturing: For the synthesis of active pharmaceutical ingredients (APIs) used in antisense oligonucleotides (ASOs) and small interfering RNA (siRNA) therapies.
• Diagnostic Probe Development: Used to create labeled or modified probes for genetic testing, fluorescence in situ hybridization (FISH), and microarray technologies.
• Research-Grade Oligo Production: Enables academic and industrial research labs to synthesize oligonucleotides with specific modifications for mechanistic studies.
• Synthesis of Modified Cytidine Analogs: The protecting group pattern allows for the introduction of cytidine derivatives with tailored properties.
• Scale-Up cGMP Manufacturing: Suitable for use in controlled, good manufacturing practice (GMP) environments for clinical and commercial therapeutic production.

Basic Information

Item	Details
Product Name	5'-O-(4,4-Dimethoxytrityl)-2'-O-[(tert-Butyl)dimethylsilyl]-N-[[4-(tert-Butyl)phenoxy]acetyl]cytidine-3'-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite
CAS No.	149989-66-2
Molecular Formula	C58H77N5O10PSi
Molecular Weight	1080.31 g/mol
Synonyms	5'-O-DMT-2'-O-TBDMS-N-Pac-Cytidine CE Phosphoramidite; N-Pac-Cytidine Phosphoramidite; Cytidine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-2'-O-[[(1,1-dimethylethyl)dimethylsilyl]-N-[2-[[4-(1,1-dimethylethyl)phenoxy]acetyl]-, 3'-[2-[[bis(1-methylethyl)amino](2-cyanoethoxy)phosphino]oxy]propyl ester; dC(Pac) Phosphoramidite; N-Phenoxyacetyl Cytidine Phosphoramidite; 2'-O-TBDMS-N-Pac-Cytidine-3'-CE Phosphoramidite
EINECS	Contact for details

Quality Control

Every batch of our phosphoramidite monomers undergoes rigorous analytical testing to ensure the high purity and consistency required for sensitive oligonucleotide synthesis. Quality is verified by HPLC, 31P NMR, and 1H NMR to confirm chemical identity, phosphitylation efficiency, and the absence of critical impurities. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and moisture content is provided with each shipment. Our production adheres to strict internal standards suitable for research and GMP-grade applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. This compound is hygroscopic (moisture-sensitive) and easily oxidized; prolonged exposure to air or moisture will degrade product quality and performance. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Retention time corresponds to reference standard
Identification (31P NMR)	Spectrum corresponds to reference standard
Purity (HPLC)	≥ 98.0%
Assay	≥ 97.0%
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.