Description

5-((4-Azidophenacyl)Thio)Cytidine-5'-Triphosphate is a chemically modified nucleotide triphosphate analog designed for advanced biochemical research and development. This compound is a critical reagent for photoaffinity labeling and cross-linking studies, enabling the covalent tagging and identification of biomolecular interactions. It is primarily utilized by researchers and manufacturers in the fields of molecular biology, enzymology, and pharmaceutical discovery who require precise tools for probing nucleic acid-protein interactions and enzyme mechanisms.

Application

• Photoaffinity Labeling: Acts as a key substrate for the covalent cross-linking and identification of proteins that bind to or modify cytidine nucleotides.
• Enzyme Mechanism Studies: Used to investigate the active sites and catalytic mechanisms of kinases, polymerases, and other nucleotide-processing enzymes.
• Nucleic Acid Research: Enables the site-specific incorporation of photoreactive and affinity tags into RNA or DNA sequences for structural and functional analysis.
• Proteomics and Target Identification: Facilitates the discovery and validation of novel drug targets by irreversibly labeling binding partners within complex biological mixtures.
• Bioconjugation and Probe Development: Serves as a building block for synthesizing advanced diagnostic and research probes that require a photo-activatable azido group.

Basic Information

Product Name	5-((4-Azidophenacyl)Thio)Cytidine-5'-Triphosphate
CAS No.	149891-83-8
Molecular Formula	C22H25N8O14P3S
Molecular Weight	750.48 g/mol
Synonyms	5-((4-Azidophenacyl)thio)CTP; N4-Azidophenacyl-thio-CTP; 5-[(4-Azidophenacyl)thio]cytidine 5'-triphosphate; Azido-Phenacyl-thio-CTP; APT-CTP; Cytidine-5'-triphosphate, 5-((4-azidophenacyl)thio)-; 5-(4-Azidophenacylthio)cytidine triphosphate; Photoactivatable CTP analog
EINECS	Contact for details

Quality Control

Our 5-((4-Azidophenacyl)Thio)Cytidine-5'-Triphosphate is manufactured under stringent conditions to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and stability parameters. We adhere to rigorous internal quality standards to guarantee product integrity, with specifications tailored to meet the demands of biochemical and pharmaceutical research.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, it is recommended to store under an inert atmosphere and avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.