Description

Z-Ala-Ala-Asn-Amc CAS NO 149697-16-5 is a synthetic, fluorogenic peptide substrate designed for the specific detection and quantification of protease activity. This compound matters because it provides researchers and quality control labs with a highly sensitive and reliable tool for enzymatic assays, crucial for drug discovery and biochemical research. It is primarily needed by pharmaceutical R&D departments, diagnostic kit manufacturers, and academic institutions studying protease function and inhibitor screening.

Application

• Protease Activity Assays: As a fluorogenic substrate for specific serine proteases and other enzymes that cleave at the amide bond.
• Drug Discovery & Development: High-throughput screening (HTS) for identifying and characterizing novel protease inhibitors.
• Biochemical Research: Kinetic studies to determine enzyme kinetics (Km, Vmax) and inhibition constants (Ki).
• Diagnostic Development: A key component in the development of clinical diagnostic tests for diseases involving abnormal protease activity.
• Quality Control: Used in biopharmaceutical manufacturing to monitor and validate the activity of enzyme-based products or processes.
• Cell Biology Studies: Detection of intracellular protease activity in live-cell imaging and flow cytometry applications.

Basic Information

Product Name	Z-Ala-Ala-Asn-Amc
CAS No.	149697-16-5
Molecular Formula	C₂₂H₂₉N₅O₈
Molecular Weight	491.50 g/mol
Synonyms	Z-AAN-AMC; Z-Ala-Ala-Asn-7-amido-4-methylcoumarin; Benzyloxycarbonyl-Ala-Ala-Asn-AMC; N-CBZ-Ala-Ala-Asn-AMC; CBZ-Ala-Ala-Asn-AMC; Z-Ala-Ala-Asparagine-AMC; Substrate for Protease; Fluorogenic Peptide Substrate Z-AAN-AMC
EINECS	Contact for details

Quality Control

Our Z-Ala-Ala-Asn-Amc is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research and diagnostic applications. Certificates of Analysis (COA) with detailed chromatographic data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.