Description

Dpn Protein CAS NO 149347-68-2 is a high-purity, recombinant protein derived from the *Dpn* restriction-modification system, known for its specific enzymatic activity. This product is critical for advanced molecular biology applications, particularly in genetic engineering and next-generation sequencing workflows, where precision and reliability are paramount. It is an essential reagent for biotechnology research laboratories, pharmaceutical development units, and diagnostic kit manufacturers requiring stringent quality control and consistent performance.

Application

• Next-Generation Sequencing (NGS) Library Preparation: Specifically used for the selective digestion of methylated DNA, crucial for reducing host background in microbiome and metagenomic studies.
• Molecular Cloning & Genetic Engineering: Employed in restriction digestion protocols to generate compatible DNA ends for ligation, facilitating precise gene insertion and vector construction.
• Epigenetics Research: A key tool for studying DNA methylation patterns by differentially cleaving methylated versus unmethylated recognition sites.
• Diagnostic Assay Development: Integrated into proprietary kits for pathogen detection and genetic screening that rely on methylation-specific DNA analysis.
• Pharmaceutical QC & Bioprocessing: Used in quality control workflows to analyze plasmid DNA and other biotherapeutic nucleic acid products for specific methylation states.
• Synthetic Biology: Applied in the assembly of synthetic genetic circuits and pathways where controlled DNA modification is required.

Basic Information

Product Name	Dpn Protein
CAS No.	149347-68-2
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	DpnI Restriction Enzyme; DpnI Endonuclease; Deoxyribonuclease DpnI; Methylation-Dependent Restriction Enzyme DpnI; DpnI Protein; DNA Adenine Methylase-Dependent Restriction Enzyme; 149347-68-2; Restriction Enzyme DpnI from *Streptococcus pneumoniae*
EINECS	Contact for details

Quality Control

Our Dpn Protein is manufactured under stringent conditions to ensure the highest standards of purity, activity, and lot-to-lot consistency. Each batch is subjected to a comprehensive battery of tests including activity assays, purity analysis via SDS-PAGE and HPLC, and stringent endotoxin testing. A Certificate of Analysis (COA) detailing specific results for purity, specific activity, and absence of contaminating nucleases is provided with every shipment to guarantee it meets the rigorous demands of research and diagnostic applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C ± 5°C. Avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation.

Specification

Item	Specification
Appearance	Clear to slightly opalescent liquid
Identification (Activity)	Cleaves methylated DNA sequence (Gm6ATC)
Purity (SDS-PAGE)	≥ 95%
Specific Activity	≥ 50,000 units/mg protein
Endotoxin Level	< 1.0 EU/µg
Contaminating Nucleases	Not detected
Storage Buffer	10 mM Tris-HCl, 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol, pH 7.4

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.