Description

Ac-Yvad-Pna is a synthetic peptide substrate specifically designed for the study and detection of caspase-1 (ICE, Interleukin-1β Converting Enzyme) activity. This compound matters as a critical tool for researchers investigating apoptosis, pyroptosis, and inflammatory pathways, providing a reliable and sensitive means to quantify enzymatic activity. It is essential for pharmaceutical R&D, academic biochemistry laboratories, and diagnostic kit manufacturers focused on developing assays for cell death and immune response mechanisms.

Application

• Caspase-1 Activity Assays: Primary substrate for fluorometric or colorimetric detection of caspase-1 in cell lysates and purified enzyme preparations.
• Drug Discovery Screening: Used in high-throughput screening platforms to identify and characterize novel inhibitors or activators of caspase-1 for therapeutic development.
• Biochemical Research: Fundamental tool for studying the kinetics, specificity, and regulation of the ICE protease in model systems.
• Apoptosis & Pyroptosis Studies: Enables the differentiation and quantification of specific caspase activities involved in programmed cell death pathways.
• Diagnostic Development: Serves as a key component in the development of research-use-only (RUO) and clinical diagnostic kits for inflammatory diseases.
• Quality Control in Biologics: Can be utilized in QC testing for biopharmaceutical processes where caspase activity is a critical parameter.

Basic Information

Product Name	Ac-Yvad-Pna
CAS No.	149231-66-3
Molecular Formula	C30H39N7O9
Molecular Weight	641.68 g/mol
Synonyms	Ac-Tyr-Val-Ala-Asp-pNA; Ac-YVAD-p-Nitroanilide; Acetyl-Tyr-Val-Ala-Asp p-nitroanilide; Caspase-1 Substrate, Ac-YVAD-pNA; ICE Substrate IV; N-Acetyl-Tyr-Val-Ala-Asp p-nitroanilide; Interleukin-1β Converting Enzyme Substrate; N-Acetyl-L-tyrosyl-L-valyl-L-alanyl-L-aspartic acid 4-nitroanilide
EINECS	Contact for details

Quality Control

Our Ac-Yvad-Pna is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot undergoes rigorous analytical testing, including HPLC for purity verification and mass spectrometry for identity confirmation. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC & MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Assay	≥ 98% (on dry basis)
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.