Description

6-Chloro-3-Indolyl Acetate is a specialized chromogenic substrate used in biochemical and histochemical staining procedures. This compound is valued for its role as a sensitive and specific indicator for enzymatic activity, particularly for phosphatases and other hydrolases. It is an essential reagent for researchers and quality control laboratories in the life sciences, pharmaceutical, and diagnostic industries requiring precise visualization of biological processes.

Application

• Enzyme Histochemistry: Primary substrate for the detection of acid and alkaline phosphatase activity in tissue sections, providing a vivid indigo-colored precipitate.
• Molecular Biology: Used in blotting techniques (e.g., Western, Southern) for non-radioactive detection of nucleic acids or proteins conjugated with reporter enzymes.
• Diagnostic Kits: A key component in clinical and veterinary diagnostic test kits for enzymatic assays.
• Microbiology: Employed in chromogenic agar media for the identification and differentiation of specific bacterial colonies based on enzyme expression.
• Plant Biology Research: Utilized in studies of plant physiology and pathology to localize enzymatic activity.
• Biochemical Research: Serves as a tool for in vitro enzyme kinetics studies and inhibitor screening assays.

Basic Information

Product Name	6-Chloro-3-Indolyl Acetate
CAS No.	149231-54-9
Molecular Formula	C10H8ClNO2
Molecular Weight	209.63 g/mol
Synonyms	6-Chloro-3-indolyl acetate; 6-Chloroindol-3-yl acetate; 3-Indolyl 6-chloroacetate; 6-Chloro-1H-indol-3-yl acetate; BCIP Acetate (common name); 5-Chloro-3-indolyl acetate (alternate numbering); X-Acetate (in some chromogenic contexts); 149231-54-9
EINECS	Contact for details

Quality Control

Our 6-Chloro-3-Indolyl Acetate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, identity confirmation by IR and NMR spectroscopy, and tests for residual solvents and moisture content. We provide comprehensive Certificates of Analysis (COA) with each shipment, ensuring traceability and compliance with your research or production standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the container to reach room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	Contact for details
Loss on Drying	≤ 1.0%
Heavy Metals	≤ 20 ppm
Solubility	Soluble in DMF, DMSO; slightly soluble in ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.