Description

5'-Guanylylimidodiphosphate Trisodium is a high-purity, non-hydrolyzable GTP analog used extensively in biochemical and pharmacological research. Its core value lies in its ability to irreversibly activate G-proteins, making it an essential tool for studying signal transduction pathways, enzyme kinetics, and receptor-ligand interactions. This compound is critically needed by researchers and manufacturers in the pharmaceutical, biotechnology, and life sciences sectors for drug discovery and fundamental molecular biology studies.

Application

• G-protein Activation Studies: Used as a potent, non-hydrolyzable agonist to lock Gα subunits in their active state for structural and functional analysis.
• Enzyme Kinetics & Inhibition Assays: Serves as a substrate analog to investigate the mechanisms of GTPases, kinases, and other nucleotide-binding enzymes.
• Membrane Receptor Research: Essential for characterizing G-protein-coupled receptors (GPCRs) and their downstream signaling cascades in vitro.
• Drug Discovery Screening: Employed in high-throughput screening platforms to identify novel modulators of GTP-binding protein activity.
• Structural Biology: Used in X-ray crystallography and cryo-EM to stabilize the active conformation of target proteins.
• Diagnostic Kit Development: A key component in diagnostic assays that monitor GTPase activity or related metabolic pathways.

Basic Information

Product Name	5'-Guanylylimidodiphosphate Trisodium
CAS No.	148892-91-5
Molecular Formula	C10H13N6O14P3Na3
Molecular Weight	603.09 g/mol
Synonyms	Guanosine 5'-[β,γ-imido]triphosphate trisodium salt; Gpp(NH)p trisodium salt; 5'-Guanylyl imidodiphosphate trisodium salt; GDPNP trisodium salt; Guanylyl-imidodiphosphate Na3; Non-hydrolyzable GTP analog trisodium salt; GMP-PNP trisodium salt; β,γ-Imido-GTP trisodium salt
EINECS	Contact for details

Quality Control

Our 5'-Guanylylimidodiphosphate Trisodium is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are provided to ensure the material meets the stringent requirements for research and diagnostic applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive) and must be kept in a dry environment. Allow the container to reach room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Endotoxin	< 10 EU/mg
Biological Activity	Conforms in functional assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.