Description

Protein Tyrosine Phosphatase Substrate I CAS NO 148851-08-5 is a specialized biochemical reagent designed as a chromogenic substrate for the detection and study of protein tyrosine phosphatase (PTP) enzyme activity. This compound is critical for researchers and developers in life sciences, enabling precise enzymatic assays and high-throughput screening for drug discovery. It is primarily utilized by pharmaceutical R&D teams, academic research laboratories, and diagnostic kit manufacturers focused on signal transduction pathways, metabolic disorders, and oncology research.

Application

• Enzymatic Assay Development: Used as a key chromogenic substrate in colorimetric and spectrophotometric assays to measure PTP activity.
• High-Throughput Screening (HTS): Essential for screening compound libraries to identify potential PTP inhibitors in drug discovery pipelines.
• Biochemical Research: Facilitates the study of phosphorylation/dephosphorylation mechanisms in cellular signaling pathways.
• Diagnostic Reagent Formulation: Incorporated into diagnostic kits for research into conditions linked to PTP dysregulation, such as diabetes and cancer.
• Enzyme Kinetics Studies: Employed to determine kinetic parameters (Km, Vmax) of various protein tyrosine phosphatases.
• Quality Control for Enzyme Production: Serves as a standard substrate for the functional validation and QC of recombinant PTP enzymes.

Basic Information

Product Name	Protein Tyrosine Phosphatase Substrate I
CAS No.	148851-08-5
Molecular Formula	C18H21N2O14P3
Molecular Weight	582.29 g/mol
Synonyms	OMPT; O-Methylfluorescein Phosphate Trisodium Salt; 3'-O-Methylfluorescein Phosphate Trisodium Salt; 6-(3'-O-Methylfluorescein) Phosphate Trisodium Salt; OMFP Trisodium Salt; PTP Substrate I; Fluorogenic PTP Substrate; 3'-O-Methylfluorescein-6-phosphate, Trisodium Salt
EINECS	Contact for details

Quality Control

Our Protein Tyrosine Phosphatase Substrate I is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity verification and spectroscopic analysis for identity confirmation, to ensure it meets the high standards required for sensitive biochemical applications. Certificates of Analysis (COA) with detailed chromatographic data are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. Due to its hygroscopic (moisture-sensitive) nature, the container should be allowed to equilibrate to room temperature before opening to prevent condensation. For long-term stability, store desiccated.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (HPLC)	Conforms
Identification (IR)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water, DMSO
Extinction Coefficient	Provided on COA
Functional Activity	≥ 90% of reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.