Description

Gal Beta(1-3)Glcnac Beta(1-3)Gal Beta(1-4)Glc-β-Pnp is a chemically defined, high-purity synthetic oligosaccharide derivative, specifically a p-nitrophenyl (PNP) glycoside. This compound serves as a critical chromogenic substrate for the specific detection and kinetic analysis of glycosidase and glycosyltransferase enzymes. It is an essential tool for researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic reagent development sectors, enabling precise biochemical assays and enzyme characterization.

Application

• Enzyme Substrate for Glycosidase Assays: Used as a specific chromogenic substrate for β-galactosidases and related enzymes, where cleavage releases p-nitrophenol for spectrophotometric detection.
• Glycosyltransferase Activity Studies: Employed in research to study the activity and specificity of glycosyltransferases involved in glycoconjugate biosynthesis.
• Diagnostic Reagent Development: A key component in the formulation of kits and reagents for clinical diagnostics, particularly for enzyme deficiency tests.
• Biochemical Research Tool: Utilized in academic and industrial R&D for investigating carbohydrate-protein interactions, enzyme mechanisms, and metabolic pathways.
• Quality Control in Biologics: Applied in the QC testing of therapeutic enzymes and other biologics to ensure batch-to-batch consistency and activity.
• High-Throughput Screening (HTS): Suitable for use in HTS platforms to identify enzyme inhibitors or activators for drug discovery programs.

Basic Information

Product Name	Gal Beta(1-3)Glcnac Beta(1-3)Gal Beta(1-4)Glc-β-Pnp
CAS No.	148705-09-3
Molecular Formula	C32H48N2O23
Molecular Weight	828.73 g/mol
Synonyms	p-Nitrophenyl β-D-galactopyranosyl-(1→3)-2-acetamido-2-deoxy-β-D-glucopyranosyl-(1→3)-β-D-galactopyranosyl-(1→4)-β-D-glucopyranoside; Galβ(1-3)GlcNAcβ(1-3)Galβ(1-4)Glcβ-PNP; PNP-Galβ1-3GlcNAcβ1-3Galβ1-4Glc; Lacto-N-tetraose derivative PNP-glycoside; Chromogenic LNT tetrasaccharide substrate; 4-Nitrophenyl lacto-N-tetraoside; Glycosidase substrate 148705-09-3
EINECS	Contact for details

Quality Control

Every batch of our Gal Beta(1-3)Glcnac Beta(1-3)Gal Beta(1-4)Glc-β-Pnp is manufactured and tested under a strict quality management system. We guarantee high purity and batch-to-batch consistency through comprehensive analytical techniques including HPLC, NMR, and MS. A detailed Certificate of Analysis (COA) providing identity, purity, and impurity profiles is supplied with each shipment to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Specific Rotation	Contact for details
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.