Description

6-(Fluorescein-5-Carboxamido)Hexanoic Acid, Succinimidyl Ester is a high-purity, amine-reactive fluorescent labeling reagent. This compound is critical for creating stable, fluorescent conjugates with proteins, peptides, and other biomolecules, enabling sensitive detection and analysis. It is primarily needed by researchers and manufacturers in the biotechnology, diagnostics, and pharmaceutical development sectors for applications in flow cytometry, immunoassays, and fluorescence microscopy.

Application

• Protein & Antibody Labeling: Conjugation to primary amines (e.g., lysine residues) for creating fluorescent probes used in immunofluorescence (IF), Western blotting, and ELISA.
• Peptide Tagging: Fluorescent labeling of synthetic peptides for tracking, cellular uptake studies, and receptor binding assays.
• Flow Cytometry & Cell Analysis: Preparation of fluorescent antibody conjugates for cell surface marker detection and intracellular staining.
• Microscopy & Imaging: Synthesis of fiducial markers and specific-target imaging agents for confocal and super-resolution microscopy.
• Diagnostic Assay Development: Key component in the manufacture of fluorescent lateral flow assays and other in vitro diagnostic (IVD) kits.
• Biomaterial & Nanoparticle Functionalization: Attaching fluorescent tags to polymers, dendrimers, or nanoparticle surfaces for tracking and biodistribution studies.

Basic Information

Product Name	6-(Fluorescein-5-Carboxamido)Hexanoic Acid, Succinimidyl Ester
CAS No.	148356-00-7
Molecular Formula	C35H27N2O10
Molecular Weight	635.60 g/mol
Synonyms	5-FAM, SE; 6-(Fluorescein-5-(N-(5-Carboxypentyl)acetamide)) Hexanoic Acid, Succinimidyl Ester; Fluorescein-5-EX, Succinimidyl Ester; 5-Carboxyfluorescein, Succinimidyl Ester, 6-isomer; 6-((5-Carboxyfluorescein)acetamido)hexanoic Acid, N-Hydroxysuccinimide Ester; SFX; 5-FAM NHS Ester; Fluorescein NHS Ester C5
EINECS	Contact for details

Quality Control

Our 6-(Fluorescein-5-Carboxamido)Hexanoic Acid, Succinimidyl Ester is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive life science applications. Certificates of Analysis (COA) with detailed chromatographic data are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC)	Conforms
Purity (HPLC)	≥95.0%
Absorption Maximum	492 nm ± 2 nm
Emission Maximum	518 nm ± 2 nm
Solubility	Soluble in DMF, DMSO, acetonitrile
Water Content (KF)	≤1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.