Description

Valyl-Leucyl-Lysyl-4-Aminomethylcoumarin is a specialized fluorogenic peptide substrate designed for the sensitive detection and study of protease activity. This compound is critical for researchers and developers requiring precise, real-time enzymatic assays in biochemical and pharmaceutical applications. It is primarily utilized in life science research, diagnostic kit development, and high-throughput screening for drug discovery.

Application

• Protease Activity Assays: As a fluorogenic substrate for monitoring the kinetics and inhibition of specific proteases, including trypsin, plasmin, and related serine proteases.
• Biochemical Research: Used in fundamental research to study enzyme mechanisms, substrate specificity, and protein-protein interactions.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors as potential therapeutic agents.
• Diagnostic Development: Serves as a key component in the development of fluorometric diagnostic tests and kits for clinical and research laboratories.
• Cell Biology Studies: Applied in live-cell imaging and assays to visualize and quantify protease activity within cellular environments.

Basic Information

Product Name	Valyl-Leucyl-Lysyl-4-Aminomethylcoumarin
CAS No.	148168-23-4
Molecular Formula	C30H47N7O7
Molecular Weight	617.75 g/mol
Synonyms	Val-Leu-Lys-AMC; VLK-AMC; H-Val-Leu-Lys-AMC; Valyl-leucyl-lysyl-7-amino-4-methylcoumarin; Fluorogenic Substrate VLK-AMC; AMC Substrate VLK; Protease Substrate 148168-23-4; (S)-2-((S)-2-((S)-2-Amino-3-methylbutanamido)-4-methylpentanamido)-6-((4-methyl-2-oxo-2H-chromen-7-yl)amino)hexanoic acid
EINECS	Contact for details

Quality Control

Our Valyl-Leucyl-Lysyl-4-Aminomethylcoumarin is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure high purity and consistent performance in sensitive fluorometric applications. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.