Description

4-Nitrophenyl2-o-(A-D-Glucopyranosyl)-A-D-Glucopyranoside is a specialized synthetic glycoside derivative, primarily used as a chromogenic substrate for enzymatic assays. This compound is critical for research and quality control in biochemistry and life sciences, enabling the sensitive and quantitative detection of specific glycosidase enzyme activity. It is an essential reagent for pharmaceutical development, diagnostic kit manufacturing, and academic research focused on carbohydrate metabolism and enzyme kinetics.

Application

• Enzymatic Substrate for Glycosidases: Acts as a chromogenic substrate for α-glucosidase and related enzymes, producing a yellow-colored 4-nitrophenol upon hydrolysis for spectrophotometric quantification.
• Pharmaceutical Research & Development: Used in high-throughput screening (HTS) assays to identify and characterize potential inhibitors for metabolic disorders like diabetes.
• Diagnostic Reagent Manufacturing: A key component in the production of clinical diagnostic kits for measuring enzyme activity in biological samples.
• Biochemical Research: Employed in kinetic studies, enzyme purification, and characterization of carbohydrate-active enzymes (CAZymes).
• Quality Control in Biotechnology: Utilized for the potency testing and batch consistency verification of enzyme preparations.
• Food & Agricultural Science: Applied in research to analyze enzyme activities relevant to food processing, preservation, and plant biochemistry.

Basic Information

Product Name	4-Nitrophenyl2-o-(A-D-Glucopyranosyl)-A-D-Glucopyranoside
CAS No.	147103-31-9
Molecular Formula	C18H25NO13
Molecular Weight	463.39 g/mol
Synonyms	4-Nitrophenyl α-maltoside; p-Nitrophenyl α-maltoside; PNPM; 4-Nitrophenyl α-D-maltoside; p-Nitrophenyl α-D-maltopyranoside; α-4-Nitrophenyl maltoside; 2-O-α-D-Glucopyranosyl-α-D-glucopyranoside, 4-nitrophenyl; 4-Nitrophenyl O-α-D-glucopyranosyl-(1→4)-α-D-glucopyranoside
EINECS	Contact for details

Quality Control

Our 4-Nitrophenyl α-maltoside is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, spectroscopic identification (IR, NMR), and specific optical rotation verification, to ensure it meets the high standards required for research and diagnostic applications. A Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term storage, consider desiccant in the container.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 98.0%
Specific Optical Rotation	Contact for details
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.