Description

n-(2-Aminoethyl)Maleimide Trifluoroacetate is a high-purity, bifunctional chemical reagent featuring both a reactive maleimide group and a primary amine, activated as a trifluoroacetate salt. This compound is critically important for its role as a heterobifunctional crosslinker, enabling the precise conjugation of biomolecules such as proteins, peptides, and antibodies under mild conditions. It is an essential tool for researchers and manufacturers in bioconjugation, pharmaceutical development, and diagnostic assay production, where reliable and specific covalent bonding is required.

Application

• Bioconjugation and Protein Labeling: Used to create stable thioether bonds between maleimide groups and cysteine thiols on proteins, while the amine handle allows for further modification or attachment to surfaces.
• Antibody-Drug Conjugate (ADC) Development: Serves as a key linker precursor in the synthesis of ADCs, connecting cytotoxic drugs to monoclonal antibodies.
• Diagnostic Reagent Manufacturing: Employed in the production of enzyme-linked immunosorbent assay (ELISA) kits and other immunoassays by conjugating enzymes to detection antibodies.
• Surface Functionalization: Used to modify nanoparticles, biosensor chips, and chromatographic resins with amine-reactive groups for subsequent biomolecule immobilization.
• Peptide Synthesis and Modification: Acts as a building block or modifying agent in solid-phase peptide synthesis (SPPS) to introduce maleimide functionality.
• Polymer Chemistry: A monomer or crosslinking agent for creating functional polymers with pendant amine or maleimide groups for advanced material science applications.

Basic Information

Item	Details
Product Name	n-(2-Aminoethyl)Maleimide Trifluoroacetate
CAS No.	146474-00-2
Molecular Formula	C8H9F3N2O4
Molecular Weight	254.17 g/mol
Synonyms	N-(2-Aminoethyl)maleimide trifluoroacetate; 1-(2-Aminoethyl)-1H-pyrrole-2,5-dione trifluoroacetate; 2,5-Pyrroledione, 1-(2-aminoethyl)-, trifluoroacetate (1:1); AEM-TFA; N-(2-Aminoethyl)maleimide TFA salt; Maleimide-PEG2-amine TFA salt (related); Aminoethylmaleimide trifluoroacetate
EINECS	Contact for details

Quality Control

Our n-(2-Aminoethyl)Maleimide Trifluoroacetate is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity, suitable for research and biopharmaceutical applications. Each lot undergoes comprehensive analytical testing, including HPLC, NMR, and mass spectrometry for identity and purity confirmation. A detailed Certificate of Analysis (COA) documenting all test results is provided with every shipment to support your quality assurance and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as indicated on the label. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. Allow the sealed container to reach room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.