Description

Lacto-n-Biosidase CAS NO 146359-52-6 is a specialized glycosidase enzyme that catalyzes the hydrolysis of specific glycosidic bonds, particularly in oligosaccharides. This compound is of significant value in biochemical research and industrial biocatalysis for its role in modifying carbohydrate structures. It is primarily utilized by researchers in glycobiology, enzymology, and by manufacturers in the food, nutraceutical, and pharmaceutical industries for the synthesis or analysis of complex carbohydrates and glycoconjugates.

Application

• Glycobiology Research: A critical tool for the structural elucidation and functional study of glycoproteins, glycolipids, and oligosaccharides.
• Enzymatic Synthesis: Used in the biocatalytic production of specific human milk oligosaccharides (HMOs) and other bioactive carbohydrates.
• Pharmaceutical Development: Employed in the modification of drug carriers or the synthesis of carbohydrate-based drug candidates.
• Diagnostic Reagent Manufacturing: Serves as a component in enzymatic assay kits for analyzing carbohydrate metabolism or related disorders.
• Food Science & Nutraceuticals: Applied in the research and development of prebiotic ingredients and functional foods.
• Biochemical Standard: Used as a reference standard in analytical laboratories for method validation and quality control.

Basic Information

Product Name	Lacto-n-Biosidase
CAS No.	146359-52-6
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	LNB-ase; β-D-Galactosidase (specific to lacto-N-biose); 1,4-β-D-Galactosyl-N-acetylglucosaminidase; Lacto-N-biosidase; Glycosidase from *Bifidobacterium bifidum*; Oligosaccharide-specific glycosyl hydrolase; EC 3.2.1.- (specific number contact for details)
EINECS	Contact for details

Quality Control

Our Lacto-n-Biosidase is produced and tested under stringent quality management systems. Each batch is characterized for specific activity, purity, and absence of contaminating enzymatic activities. A comprehensive Certificate of Analysis (COA) is provided, detailing parameters such as protein concentration, activity units, and stability data. We adhere to relevant guidelines for research-grade biochemicals to ensure batch-to-batch consistency and reliable performance.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This material is hygroscopic (moisture-sensitive); ensure containers are sealed tightly after use to minimize exposure to atmospheric moisture. For optimal activity retention, avoid repeated freeze-thaw cycles by aliquoting into single-use portions.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (IR / Activity)	Conforms
Specific Activity	> X units/mg protein (Contact for details)
Protein Content (Bradford)	> 90%
Purity (SDS-PAGE)	Single major band
pH (for solutions)	6.5 - 7.5
Endotoxin Level	< 1.0 EU/mg (if required)
Storage Buffer	Specified phosphate or Tris buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.