Description

Cotinine-n-(4-Deoxy-4,5-Didehydro)--D-Glucuronide is a high-purity, chemically defined metabolite of cotinine, a primary biomarker for nicotine exposure. This compound is critical for research and diagnostic applications requiring precise quantification and validation. It is primarily utilized by analytical laboratories, diagnostic kit manufacturers, and pharmaceutical research institutions focused on tobacco-related studies, toxicology, and biomarker assay development.

Application

• Reference Standard for Analytical Chemistry: Used as a certified reference material (CRM) for the calibration and validation of analytical methods, including LC-MS/MS and HPLC, in clinical and forensic toxicology.
• Diagnostic Assay Development: Serves as a key antigen or hapten in the development and quality control of immunoassays (ELISA, RIA) for detecting nicotine and cotinine exposure in biological samples.
• Pharmacokinetic & Metabolic Studies: Employed in research to study the glucuronidation pathway, metabolism, and elimination kinetics of cotinine in both preclinical and clinical settings.
• Biomarker Research: Essential for investigating the correlation between tobacco use, disease risk, and exposure levels in epidemiological and public health studies.
• Quality Control in Clinical Laboratories: Used as an internal standard or control material to ensure the accuracy and reliability of routine testing for nicotine metabolites in urine, blood, or saliva.
• Regulatory & Compliance Testing: Supports workplace drug testing programs and smoking cessation studies that require precise and legally defensible analytical results.

Basic Information

Product Name	Cotinine-n-(4-Deoxy-4,5-Didehydro)--D-Glucuronide
CAS No.	146275-15-2
Molecular Formula	C₁₆H₂₂N₂O₇
Molecular Weight	354.36 g/mol
Synonyms	Cotinine N-Glucuronide (4,5-unsaturated); (E)-Cotinine N-β-D-Glucopyranosiduronic Acid (4-Deoxy-4,5-didehydro); trans-3'-Hydroxycotinine O-β-D-Glucuronide (isomer); Cotinine Glucuronide Metabolite; Nicotine Metabolite Reference Standard; 1-((2E)-1-Methyl-5-(3-pyridinyl)-2-pyrrolidinyl)-β-D-glucopyranosiduronic Acid; 146275-15-2
EINECS	Contact for details

Quality Control

Our Cotinine-n-(4-Deoxy-4,5-Didehydro)--D-Glucuronide is manufactured under strict quality management systems. Each batch is subjected to rigorous identity, purity, and impurity profile testing using advanced analytical techniques such as NMR, HPLC, and mass spectrometry to ensure it meets the high standards required for reference materials and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, chromatographic data, and storage recommendations is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry, cool place. This product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccation. Allow the vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.