Description

n-Succinimidyl 4-Hydroxy-3-Iodobenzoate is a highly reactive, heterobifunctional crosslinking agent designed for the site-specific conjugation of biomolecules. Its core value lies in enabling the stable and efficient labeling of proteins, peptides, and other amine-containing compounds with tags, probes, or solid supports. This compound is essential for researchers and manufacturers in bioconjugation, diagnostic assay development, and pharmaceutical research, particularly for creating targeted imaging agents and antibody-drug conjugates (ADCs).

Application

• Bioconjugation Reagent: Primary use for covalently linking amine-containing biomolecules (e.g., antibodies, peptides) to various functional groups, fluorophores, or solid phases.
• Diagnostic Kit Manufacturing: Critical for the production of enzyme-linked immunosorbent assays (ELISAs), lateral flow assays, and other immunoassays by immobilizing capture antibodies or antigens.
• Antibody-Drug Conjugate (ADC) Development: Serves as a key linker intermediate in the synthesis of ADCs, connecting cytotoxic payloads to monoclonal antibodies.
• Protein Labeling & Tagging: Used to attach biotin, fluorescent dyes (e.g., Cy dyes, FITC), or other reporter molecules to proteins for detection and purification.
• Surface Functionalization: Enables the modification of biosensor chips, microarray slides, and nanoparticles with bioactive ligands for research and diagnostic platforms.
• Peptide Synthesis & Modification: Employed in solid-phase peptide synthesis (SPPS) to introduce specific handles or labels onto synthetic peptides.

Basic Information

Product Name	n-Succinimidyl 4-Hydroxy-3-Iodobenzoate
CAS No.	145459-41-2
Molecular Formula	C11H8INO5
Molecular Weight	361.09 g/mol
Synonyms	4-Hydroxy-3-iodobenzoic Acid N-Hydroxysuccinimide Ester; 3-Iodo-4-hydroxybenzoic Acid N-Succinimidyl Ester; NHS-Iodohydroxybenzoate; SIB (Succinimidyl Iodobenzoate); 4-(N-Succinimidyloxycarbonyl)-2-iodophenol; N-Succinimidyl 3-Iodo-4-hydroxybenzoate; 145459-41-2; Iodo-Hydroxybenzoic Acid NHS Ester
EINECS	Contact for details

Quality Control

Our n-Succinimidyl 4-Hydroxy-3-Iodobenzoate is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity, critical for sensitive bioconjugation workflows. Each lot undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry. A detailed Certificate of Analysis (COA) providing full specification results is supplied with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent hydrolysis and loss of reactivity. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.