Description

Biotin-36-Dctp CAS NO 145396-05-0 is a high-purity, modified nucleotide triphosphate (dCTP) analog, specifically engineered for advanced molecular biology and diagnostic applications. This compound is critical for researchers and developers requiring precise, non-radioactive labeling and detection in nucleic acid analysis. It is primarily utilized in the life sciences sector for applications such as DNA sequencing, microarray fabrication, and in vitro diagnostics.

Application

• DNA Sequencing & Labeling: Acts as a substrate for DNA polymerases in next-generation sequencing (NGS) and Sanger sequencing workflows for the incorporation of biotin labels.
• Microarray & Chip Manufacturing: Used in the synthesis of biotinylated probes for immobilization on microarray slides and biosensor chips.
• In Vitro Diagnostics (IVD): A key reagent in the development of PCR-based diagnostic kits and assays for pathogen detection and genetic testing.
• Fluorescence In Situ Hybridization (FISH): Enables the preparation of non-radioactive, biotin-tagged nucleic acid probes for chromosomal and genetic analysis.
• Pull-down Assays & Affinity Purification: Facilitates the biotinylation of DNA fragments for streptavidin-based capture and purification of protein-DNA complexes.
• Single Nucleotide Polymorphism (SNP) Detection: Employed in genotyping assays that require highly specific nucleotide incorporation and detection.

Basic Information

Product Name	Biotin-36-Dctp
CAS No.	145396-05-0
Molecular Formula	C₂₀H₃₃N₆O₁₅P₃S
Molecular Weight	722.49 g/mol
Synonyms	Biotin-16-ddCTP; Biotin-16-2',3'-Dideoxycytidine-5'-Triphosphate; 2',3'-Dideoxycytidine-5'-triphosphate, 5'-[16-[(3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]-4,7,10,13-tetraoxahexadecan-1-yl] ester; Biotinylated ddCTP; Biotin-16-2',3'-Dideoxy-CTP; 5'-Biotin-16-ddCTP; N/A-(Biotin-16)-2',3'-Dideoxycytidine-5'-Triphosphate
EINECS	Contact for details

Quality Control

Every batch of Biotin-36-Dctp is manufactured under strict quality management systems and undergoes rigorous analytical testing to ensure identity, purity, and performance consistency. Our products are suitable for research and diagnostic use, with specifications typically meeting or exceeding industry requirements. A comprehensive Certificate of Analysis (COA) detailing purity (HPLC), identity (NMR/MS), and functional performance data is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer. The product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid or lyophilized powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (Karl Fischer)	≤ 3.0%
Functional Test (Enzyme Incorporation)	Suitable for sequencing

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.