Description

Exo-n-(2-(4-Azidotetrafluorobenzamido)Ethyl)-Deoxycytidine-5'-Triphosphate is a chemically modified nucleotide triphosphate analog designed for advanced bioconjugation and labeling applications. This compound is critical for researchers requiring a high-energy nucleotide substrate that can be selectively and covalently attached to biomolecules via a photoactivatable azide group. It is primarily needed by biotechnology and pharmaceutical R&D teams working in next-generation sequencing (NGS) probe development, click chemistry-based diagnostics, and the synthesis of functionalized nucleic acid libraries.

Application

• Next-Generation Sequencing (NGS): Enzymatic incorporation for the site-specific labeling of DNA during sequencing-by-synthesis workflows.
• Click Chemistry & Bioconjugation: Acts as a key reagent for photoaffinity labeling and copper-free click chemistry reactions to tag nucleic acids with fluorophores or other probes.
• Functional Genomics: Synthesis of modified primers and probes for advanced PCR and microarray applications requiring covalent attachment.
• Diagnostic Assay Development: Used in the creation of highly specific detection kits that utilize covalent nucleotide capture for enhanced sensitivity.
• Structural Biology Studies: Cross-linking agent for investigating nucleic acid-protein interactions via UV-induced cross-linking.
• Antiviral & Anticancer Research: Serves as a modified substrate for studying polymerase mechanisms and developing chain-terminating therapeutic agents.

Basic Information

Product Name	Exo-n-(2-(4-Azidotetrafluorobenzamido)Ethyl)-Deoxycytidine-5'-Triphosphate
CAS No.	145278-12-2
Molecular Formula	C₁₉H₂₁F₄N₈O₁₃P₃
Molecular Weight	786.36 g/mol
Synonyms	4-Azido-2,3,5,6-tetrafluorobenzoyl-EDA-dCTP; N-(2-((4-Azido-2,3,5,6-tetrafluorobenzoyl)amino)ethyl)-2'-deoxycytidine-5'-triphosphate; Azido-tetrafluorobenzoyl-ethylenediamine-dCTP; exo-N-(2-(4-Azidotetrafluorobenzamido)ethyl)-2'-deoxycytidine-5'-triphosphate; Photoactivatable dCTP; TFPA-EDA-dCTP; Azido-PFPA-dCTP; Modified Nucleotide Triphosphate 145278-12-2
EINECS	Contact for details

Quality Control

Our production of this high-purity biochemical adheres to stringent quality protocols. Each batch is characterized and tested to ensure >95% purity (HPLC) and the absence of contaminating nucleases and phosphatases. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing specifications for identity (NMR/IR), purity, and functional performance in enzymatic incorporation assays.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and strictly light-sensitive (protect from all light exposure). Aliquot to avoid repeated freeze-thaw cycles. Keep under inert atmosphere for long-term storage.

Specification

Item	Specification
Appearance	White to off-white solid or lyophilized powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 3.0%
Enzymatic Incorporation	Functional in standard polymerase assays
Solubility	Soluble in aqueous buffers, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.