Description

4-(4-(2-(4-(N,n-Dimethylamino)Phenyl)Ethenyl)Pyridinio)Butyrate n-Hydroxysuccinimide Ester is a specialized, high-purity chemical reagent designed for advanced bioconjugation and labeling applications. Its core value lies in its dual functionality, combining a reactive N-hydroxysuccinimide (NHS) ester group for efficient amine coupling with a dimethylaminostyryl pyridinium moiety that serves as a fluorescent or photoactive label. This compound is essential for researchers and manufacturers in the life sciences sector who require reliable, high-performance reagents for developing diagnostic assays, fluorescent probes, and targeted drug delivery systems.

Application

• Fluorescent Labeling of Biomolecules: Used to covalently attach a fluorescent tag to proteins, antibodies, peptides, and oligonucleotides for detection and imaging.
• Diagnostic Assay Development: Serves as a key component in the synthesis of conjugates for ELISA, lateral flow assays, and other immunoassay platforms.
• Bioimaging Probe Synthesis: Employed in the creation of cell-permeable fluorescent dyes for live-cell imaging and microscopy studies.
• Photodynamic Therapy (PDT) Research: Acts as a potential photosensitizer precursor due to its light-absorbing properties.
• Polymer and Material Science: Used to functionalize surfaces, nanoparticles, and polymers with amine-reactive, fluorescent groups.
• Chemical Biology Tool Development: Facilitates the study of protein interactions and localization through site-specific labeling.

Basic Information

Product Name	4-(4-(2-(4-(N,n-Dimethylamino)Phenyl)Ethenyl)Pyridinio)Butyrate n-Hydroxysuccinimide Ester
CAS No.	145128-16-1
Molecular Formula	C24H26N3O4+ • X-
Molecular Weight	420.48 g/mol (for cation)
Synonyms	NHS-DMASP; DMASP-NHS Ester; 4-[4-[2-[4-(Dimethylamino)phenyl]ethenyl]-1-pyridinio]butanoate 2,5-dioxo-1-pyrrolidinyl ester; 4-(4-(2-(4-(Dimethylamino)styryl)pyridinium-1-yl)butanoic acid N-hydroxysuccinimide ester; DMASP NHS ester; Pyridinium, 4-[2-[4-(dimethylamino)phenyl]ethenyl]-1-[4-[(2,5-dioxo-1-pyrrolidinyl)oxy]-4-oxobutyl]-, inner salt; CAS 145128-16-1
EINECS	Contact for details

Quality Control

Our 4-(4-(2-(4-(N,n-Dimethylamino)Phenyl)Ethenyl)Pyridinio)Butyrate n-Hydroxysuccinimide Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and spectrophotometry to verify key optical properties. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing all relevant specifications to ensure traceability and compliance with your research or production requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant (e.g., in a sealed container with silica gel) to minimize hydrolysis of the NHS ester group and degradation due to its hygroscopic nature. For long-term stability, store under inert atmosphere (argon or nitrogen). Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and moisture ingress.

Specification

Item	Specification
Appearance	Red to dark red solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	~ 480 - 520 nm
Extinction Coefficient (ε)	Contact for details
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.