Description

H-Ala-Phe-Pna Hcl is a chemically synthesized peptide derivative, specifically a tripeptide para-nitroanilide (Pna) substrate. This compound is a high-purity biochemical reagent valued for its role as a selective and sensitive chromogenic substrate for enzymatic activity studies. It is primarily utilized by research institutions and pharmaceutical companies engaged in protease and peptidase research, high-throughput screening, and biochemical assay development.

Application

• Protease/Peptidase Research: Acts as a chromogenic substrate for kinetic studies and characterization of enzyme specificity and inhibition.
• High-Throughput Screening (HTS): Used in pharmaceutical discovery to identify and optimize novel protease inhibitors for therapeutic targets.
• Biochemical Assay Development: Serves as a key component in developing colorimetric assays to measure enzymatic activity in complex biological samples.
• Enzyme Mechanism Studies: Employed to investigate the catalytic mechanisms and cleavage preferences of various serine, cysteine, or metallo-proteases.
• Diagnostic Reagent Manufacturing: Can be incorporated into diagnostic kits for detecting specific protease activities associated with diseases.
• Academic & Industrial R&D: Supports fundamental and applied research in biochemistry, molecular biology, and medicinal chemistry.

Basic Information

Item	Detail
Product Name	H-Ala-Phe-Pna Hcl
CAS No.	145090-14-8
Molecular Formula	C20H23N5O5 • HCl
Molecular Weight	457.89 g/mol (Free base: 421.43 g/mol)
Synonyms	Ala-Phe-pNA • H-Ala-Phe-p-Nitroanilide Hydrochloride • L-Alanyl-L-phenylalanine 4-nitroanilide hydrochloride • Substrate for Proteases • APpNA • AAF-pNA • N-Succinyl-Ala-Ala-Phe-p-nitroanilide related substrate • Peptide p-nitroanilide substrate
EINECS	Contact for details

Quality Control

Our H-Ala-Phe-Pna Hcl is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and specific tests for residual solvents and moisture content. A Certificate of Analysis (COA) detailing all specifications is provided with every shipment to ensure traceability and compliance with your research-grade requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive); allow the sealed vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For short-term use, store desiccated at 2-8°C.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Mass spectrum consistent with structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.