Description

Rhod-2, Am is a cell-permeant, acetoxymethyl (AM) ester derivative of the calcium indicator Rhod-2, designed for intracellular calcium imaging. This compound is crucial for researchers requiring high-affinity, long-wavelength detection of calcium transients in living cells, particularly in mitochondria. It is primarily needed by scientists and R&D professionals in the fields of cell biology, neuroscience, and pharmacology for studying calcium signaling dynamics.

Application

• Intracellular Calcium Imaging: Fluorescent detection of cytosolic and mitochondrial calcium ion (Ca²⁺) fluxes in live cells.
• Neuroscience Research: Monitoring neuronal activity and calcium signaling in synapses and cell bodies.
• Cardiovascular Research: Studying calcium handling and excitation-contraction coupling in cardiomyocytes.
• Drug Discovery & Screening: Used in high-throughput assays to evaluate compound effects on cellular calcium homeostasis.
• Mitochondrial Function Analysis: Specific targeting to mitochondria for assessing calcium uptake and its role in bioenergetics and apoptosis.
• Multiplex Fluorescence Assays: Compatible with other fluorophores due to its long excitation/emission wavelengths (~553/581 nm).

Basic Information

Product Name	Rhod-2, Am
CAS No.	145037-81-6
Molecular Formula	C₄₇H₅₀Cl₂N₄O₁₉
Molecular Weight	1061.82 g/mol
Synonyms	Rhod-2 acetoxymethyl ester; Rhod-2 AM ester; Rhod-2, AM; X-Rhod-1, AM (related analog); Calcium indicator Rhod-2 AM; 5-(and-6)-Carboxy-X-rhodamine, acetoxymethyl ester, triethylammonium salt; Tetramethylrhodamine-based Ca²⁺ indicator AM ester
EINECS	Contact for details

Quality Control

Every batch of Rhod-2, Am is manufactured and tested under strict quality protocols to ensure high purity and consistent performance for sensitive research applications. Our products undergo rigorous quality testing, including HPLC analysis for purity and identity confirmation. Certificates of Analysis (COA) detailing batch-specific results are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	Orange to red solid or film
Identification (HPLC/UV-Vis)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF
Functional Activity (Cell-based assay)	Conforms to reference

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.