Description

1-Hydroxy Alprazolam B-D-Glucuronide is a major, pharmacologically relevant metabolite of the benzodiazepine drug alprazolam. This compound is of critical importance for research and development in forensic, clinical, and pharmaceutical chemistry. It serves as a primary reference standard for the accurate quantification and confirmation of alprazolam use in biological matrices. Professionals in toxicology laboratories, pharmaceutical quality control, and clinical research require this high-purity analytical standard to ensure method validation and regulatory compliance.

Application

• Forensic Toxicology Reference Standard: Used as a certified reference material (CRM) for the identification and quantification of alprazolam and its metabolites in urine, blood, and other forensic samples.
• Clinical Research & Pharmacokinetics: Essential for studying the metabolic pathways, clearance rates, and bioavailability of alprazolam in human subjects.
• Pharmaceutical Impurity Profiling: Employed in the analysis and control of potential glucuronide conjugate impurities during alprazolam drug substance and product manufacturing.
• Doping Control & Sports Drug Testing: A key analyte for methods designed to detect benzodiazepine use in athletic competitions.
• Method Development & Validation: Serves as a critical component for developing, calibrating, and validating LC-MS/MS, HPLC, and GC-MS analytical methods.
• Clinical Laboratory Testing: Used to ensure the accuracy and precision of commercial immunoassays and chromatographic tests for therapeutic drug monitoring (TDM).

Basic Information

Product Name	1-Hydroxy Alprazolam B-D-Glucuronide
CAS No.	144964-58-9
Molecular Formula	C24H25ClN4O8
Molecular Weight	532.93 g/mol
Synonyms	α-Hydroxyalprazolam Glucuronide; Alprazolam Metabolite; 1-Hydroxy Alprazolam Glucuronide; 8-Chloro-1-methyl-6-phenyl-4H-[1,2,4]triazolo[4,3-a][1,4]benzodiazepine-1-glucuronide; 1-Hydroxyalprazolam β-D-Glucopyranosiduronic Acid; Alprazolam 1-O-β-D-Glucuronide; 1'-OH-Alprazolam Glucuronide; Xanax Metabolite
EINECS	Contact for details

Quality Control

Every batch of 1-Hydroxy Alprazolam B-D-Glucuronide is manufactured and analyzed under strict quality management systems. The product undergoes rigorous identity confirmation, purity assay, and impurity profiling using advanced chromatographic (HPLC, UPLC) and spectroscopic (MS, NMR) techniques to ensure it meets the stringent requirements for a high-purity reference standard. A comprehensive Certificate of Analysis (COA) detailing batch-specific results for identity, purity, and related substances is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature, ideally between 15-25°C (59-77°F). For long-term storage, desiccate at -20°C. The product is light-sensitive and should be handled under appropriate conditions to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Mass spectrum conforms to structure
Purity (HPLC)	≥ 98.0%
Related Substances (HPLC)	Total impurities ≤ 2.0% Any single impurity ≤ 1.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.