Description

2-(N'-Acetylphenylalanyl)Hydroxyethyl-2'-Pyridyl Disulfide is a specialized heterobifunctional crosslinking reagent featuring a protected amine-reactive group and a cleavable disulfide bond. This compound matters for its precise role in bioconjugation, enabling the controlled attachment of molecules like peptides, proteins, or labels to surfaces or other biomolecules while allowing for subsequent reductive cleavage. It is primarily needed by researchers and manufacturers in pharmaceutical development, proteomics, and advanced material science for creating sophisticated drug delivery systems, diagnostic assays, and functionalized matrices.

Application

• Bioconjugation and Protein Modification: Used as a key reagent for site-specific crosslinking of peptides, antibodies, or enzymes to solid supports, nanoparticles, or other proteins.
• Drug Delivery System Development: Facilitates the construction of cleavable linker systems in antibody-drug conjugates (ADCs) and targeted therapeutic platforms.
• Surface Functionalization: Enables the controlled immobilization of biomolecules on biosensor chips, chromatography media, and diagnostic devices.
• Proteomics Research: Employed in affinity chromatography and pull-down assays for studying protein-protein interactions and post-translational modifications.
• Chemical Biology Tool Development: Serves as a building block for synthesizing novel probes and reagents that require reversible covalent bonding.

Basic Information

Product Name	2-(N'-Acetylphenylalanyl)Hydroxyethyl-2'-Pyridyl Disulfide
CAS No.	144909-58-0
Molecular Formula	C18H21N3O4S2
Molecular Weight	407.51 g/mol
Synonyms	N-α-Acetyl-L-phenylalanyl-N-(2-hydroxyethyl)-2-pyridyldisulfide amide; Ac-Phe-HE-pyridyl disulfide; 2-(N-Acetyl-L-phenylalanylamino)ethyl 2-pyridyl disulfide; 2-[N-(N-Acetyl-L-phenylalanyl)]aminoethyl 2-pyridyl disulfide; Heterobifunctional crosslinker (Ac-Phe-HE); N-Acetylphenylalanylhydroxyethyl pyridyl disulfide; N-(2-Hydroxyethyl)-N'-(2-pyridyldithio)acetylphenylalanine amide
EINECS	Contact for details

Quality Control

Our 2-(N'-Acetylphenylalanyl)Hydroxyethyl-2'-Pyridyl Disulfide is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and specific tests for moisture and residual solvents. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with every shipment to ensure traceability and compliance with your research or production standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) to minimize oxidation and hydrolysis. Allow the container to reach room temperature in a desiccator before opening to prevent moisture condensation. Keep away from strong reducing agents.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solvent Residues (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.