Description

Glutaryl-Alanyl-Alanyl-Phenylalanyl-Amidomethylcoumarin is a specialized fluorogenic peptide substrate designed for high-sensitivity enzymatic assays. This compound is critical for researchers and quality control laboratories requiring precise detection and quantification of protease activity. It is primarily utilized in the pharmaceutical, biotechnology, and diagnostic industries for drug discovery, biochemical research, and enzyme characterization.

Application

• Protease Activity Assays: A key fluorogenic substrate for the detection and kinetic analysis of various serine and cysteine proteases.
• High-Throughput Screening (HTS): Enables rapid screening of protease inhibitors in drug discovery and development pipelines.
• Biochemical Research: Used in studying enzyme mechanisms, specificity, and inhibition constants (Ki) in academic and industrial R&D settings.
• Diagnostic Kit Development: Serves as a core component in developing sensitive diagnostic assays for diseases involving aberrant protease activity.
• Quality Control Testing: Employed in biopharmaceutical manufacturing to monitor the activity and consistency of enzyme-based products.

Basic Information

Product Name	Glutaryl-Alanyl-Alanyl-Phenylalanyl-Amidomethylcoumarin
CAS No.	144398-31-2
Molecular Formula	C34H41N5O9
Molecular Weight	663.72 g/mol
Synonyms	Glutaryl-AAPF-AMC; Glutaryl-Ala-Ala-Phe-AMC; GAAFMCA; AAPF-AMC Substrate; Fluorogenic Substrate for Proteases; Glutaryl-AAF-AMC; 7-Amino-4-methylcoumarin (AMC) derivative; Glutaryl-Ala-Ala-Phe-7-amido-4-methylcoumarin
EINECS	Contact for details

Quality Control

Our Glutaryl-Alanyl-Alanyl-Phenylalanyl-Amidomethylcoumarin is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and functional activity validation. Certificates of Analysis (COA) detailing all specifications are provided to ensure traceability and compliance with research-grade standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Functional Activity	Conforms (Specific activity available on COA)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.