Description

Fluoresceinthiocarbamoyl-Lys-Glcnac-1-4-Murnac-Ala-Isoglutamine is a sophisticated, fluorescently-labeled peptidoglycan precursor analog, a key tool compound for advanced biochemical research. Its core value lies in enabling the real-time, sensitive detection and study of bacterial cell wall biosynthesis and remodeling processes. This high-purity biochemical is essential for researchers and developers in antibiotic discovery, enzymology, and bacterial pathogenesis studies, providing critical insights into novel therapeutic targets.

Application

• Enzymatic Assay Substrate: Primary substrate for high-throughput screening (HTS) of enzymes like MurG (UDP-N-acetylglucosamine-N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase) and other peptidoglycan glycosyltransferases.
• Antibiotic Mechanism of Action Studies: Used to investigate the inhibitory effects of novel and existing antibiotics on bacterial cell wall synthesis pathways.
• Fluorescence-Based Detection: Enables sensitive, real-time monitoring of enzymatic activity in vitro without the need for radioactive labels.
• Biochemical Research Tool: Critical for studying the specificity, kinetics, and inhibition profiles of bacterial membrane-bound enzymes involved in peptidoglycan assembly.
• Drug Discovery Platforms: Integral component in assay development for pharmaceutical R&D targeting multidrug-resistant bacterial infections.
• Academic Research: Supports fundamental research in microbiology, structural biology, and chemical biology focused on bacterial cell envelope biogenesis.

Basic Information

Product Name	Fluoresceinthiocarbamoyl-Lys-Glcnac-1-4-Murnac-Ala-Isoglutamine
CAS No.	143873-62-5
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	FITC-Lys-GlcNAc-1,4-MurNAc-Ala-isoGln; Fluorescein-labeled Lipid II analog; Fluorescent UDP-MurNAc-pentapeptide derivative; Substrate for MurG transferase; Fluorescein thiocarbamoyl lysine derivative; Peptidoglycan biosynthesis fluorescent probe; Bacterial cell wall synthesis tracer
EINECS	Contact for details

Quality Control

This high-value biochemical is manufactured under strict quality control protocols to ensure batch-to-batch consistency and research reliability. Purity is rigorously verified using advanced analytical techniques including HPLC and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and performance in standard enzymatic assays is provided with each batch to support your research integrity.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	Yellow to orange solid
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95%
Assay (Enzymatic)	≥ 90% active substrate
Solubility	Soluble in DMSO, DMF
Water Content (KF)	< 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.