Description

5'-Monophosphoryladenylyl-(2'-5')Adenylyl-(2'-5')-8-Bromoadenylyl-(2'-5')-8-Bromoadenosine is a chemically modified oligonucleotide analog, specifically a 2'-5' linked tetramer incorporating brominated adenosine residues. This compound is of significant interest as a highly specific biochemical tool for probing RNA structure, function, and enzyme-substrate interactions. It is primarily utilized by research institutions and pharmaceutical R&D departments focused on antiviral mechanisms, oligonucleotide therapeutics, and fundamental nucleic acid biochemistry.

Application

• Biochemical Research: As a substrate or inhibitor for studying the activity and specificity of 2'-5'-oligoadenylate synthetases and related nucleotidyltransferases.
• Antiviral Mechanism Studies: Investigation of the 2-5A system pathway, which is part of the innate immune response to viral infection.
• Oligonucleotide Therapeutic Development: Serving as a reference compound or a modified building block in the design of novel therapeutic oligonucleotides with enhanced stability.
• Enzymology: Probing the active sites and catalytic mechanisms of enzymes that process or recognize non-canonical nucleic acid linkages.
• Structural Biology: Use in X-ray crystallography or NMR studies to determine the three-dimensional structure of protein-RNA complexes.
• Diagnostic Assay Development: Potential use as a specialized component in high-sensitivity assays for detecting specific enzymatic activities.

Basic Information

Product Name	5'-Monophosphoryladenylyl-(2'-5')Adenylyl-(2'-5')-8-Bromoadenylyl-(2'-5')-8-Bromoadenosine
CAS No.	143832-09-1
Molecular Formula	C40H48Br2N20O24P3
Molecular Weight	1444.59 g/mol
Synonyms	2'-5' ppp(A2'p)2(8BrA2'p)(8BrA); 2-5A Tetramer, 8-Bromo Modified; 8-Bromoadenosine 2',5'-Tetramer 5'-Triphosphate; ppp(A2'p)2(8BrA2'p)(8BrA); 5'-Triphosphoryl-2'-5'-adenylyl-(2'-5')-adenylyl-(2'-5')-8-bromoadenylyl-(2'-5')-8-bromoadenosine; 2',5'-Oligoadenylate Tetramer (8-Bromo Analog); P1,P3,P4-Tri(5'-adenosyl) P2-(8-bromoadenosine 5'-) tetraphosphate
EINECS	Contact for details

Quality Control

This high-purity biochemical is manufactured under controlled conditions to ensure batch-to-batch consistency for critical research applications. Quality is verified through a suite of analytical techniques including HPLC, MS, and NMR to confirm identity, purity, and the absence of undesired isomers or degradation products. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, consider aliquoting under an inert atmosphere to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Assay (UV)	≥ 90% (on anhydrous basis)
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water, buffer solutions

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.