Description

2-Azido-2'-3'-(2,2,5,5-Tetramethyl-3-Pyrroline-1-Oxyl-3-Carboxylic Acid Ester)Atp is a specialized spin-labeled ATP analog designed for advanced biophysical and biochemical research. This compound is critical for studying enzyme kinetics, nucleotide-binding interactions, and conformational dynamics using techniques like Electron Paramagnetic Resonance (EPR) spectroscopy. It is primarily utilized by research scientists and pharmaceutical developers in the fields of structural biology, enzymology, and drug discovery.

Application

• EPR Spectroscopy Studies: As a spin-labeled probe for investigating the structure and dynamics of ATP-binding proteins and enzymes.
• Enzyme Mechanism Analysis: To monitor real-time conformational changes and binding events in kinases, ATPases, and other nucleotide-dependent enzymes.
• Drug Discovery Screening: Used in assays to identify and characterize inhibitors or modulators of ATP-binding sites in therapeutic targets.
• Structural Biology: Aids in mapping binding pockets and understanding protein-ligand interactions through distance measurements.
• Biophysical Probes: Serves as a sensitive reporter for studying molecular interactions in complex biological systems.
• Nucleotide Analog Research: Enables the study of energy transfer and catalytic mechanisms in biochemical pathways.

Basic Information

Product Name	2-Azido-2'-3'-(2,2,5,5-Tetramethyl-3-Pyrroline-1-Oxyl-3-Carboxylic Acid Ester)Atp
CAS No.	143796-55-8
Molecular Formula	C₂₀H₂₈N₈O₁₃P₃
Molecular Weight	690.42 g/mol
Synonyms	2-Azido-ATP, spin-labeled; SL-ATP; 2-Azido-2'-3'-(TEMPO-3-carboxylate)ATP; ATP, 2-azido-2'-3'-[[(2,2,5,5-tetramethyl-1-oxyl-3-pyrrolidinyl)carbonyl]oxy]-; Nitrene-generating ATP analog; Spin-labeled adenosine triphosphate; EPR-ATP; 2-N₃-ATP-TEMPO ester
EINECS	Contact for details

Quality Control

Every batch of 2-Azido-2'-3'-(2,2,5,5-Tetramethyl-3-Pyrroline-1-Oxyl-3-Carboxylic Acid Ester)Atp is produced and tested under stringent quality protocols to ensure high purity and consistent performance for sensitive research applications. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing purity, identity, and impurity profiles. Our quality system is designed to meet the exacting standards of research and pharmaceutical development.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccation and storage under an inert atmosphere. Allow the product to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
EPR Signal	Active
Solubility	Soluble in DMSO, buffer solutions

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.