Description

Z-Gly-Pro-Arg-4-Methoxy-2-Naphthylamine is a specialized fluorogenic peptide substrate designed for the sensitive detection of serine proteases, most notably thrombin. Its core value lies in its ability to release a highly fluorescent 4-methoxy-2-naphthylamine (MNA) moiety upon enzymatic cleavage, enabling precise and quantitative activity measurements. This compound is an essential tool for researchers and quality control laboratories in the pharmaceutical, diagnostic, and life science sectors, particularly for studying coagulation pathways, developing enzyme inhibitors, and conducting high-throughput screening assays.

Application

• Thrombin Activity Assays: Primary substrate for the specific and sensitive fluorometric determination of thrombin activity in research and diagnostic applications.
• Coagulation Pathway Research: Used to study the kinetics and inhibition of key serine proteases involved in the blood coagulation cascade.
• High-Throughput Screening (HTS): Enables the rapid screening of potential anticoagulant and antithrombotic drug candidates in pharmaceutical development.
• Enzyme Characterization: A tool for characterizing the specificity and catalytic efficiency of trypsin-like proteases.
• Diagnostic Kit Component: Serves as a critical reagent in the formulation of in-vitro diagnostic (IVD) kits for hemostasis testing.
• Biochemical Research: Employed in general protease research to investigate enzymatic mechanisms and protein-protein interactions.

Basic Information

Product Name	Z-Gly-Pro-Arg-4-Methoxy-2-Naphthylamine
CAS No.	143180-73-8
Molecular Formula	C33H41N7O7
Molecular Weight	647.73 g/mol
Synonyms	Boc-Gly-Pro-Arg-MNA; Z-Gly-Pro-Arg-MNA; Benzyloxycarbonyl-Gly-Pro-Arg-4-Methoxy-2-Naphthylamide; Thrombin Substrate IV; Fluorogenic Thrombin Substrate; MCA-Gly-Pro-Arg-MNA (common alternative naming); Boc-GPR-MNA; Z-GPR-MNA
EINECS	Contact for details

Quality Control

Every batch of Z-Gly-Pro-Arg-4-Methoxy-2-Naphthylamine is manufactured and tested under strict quality management systems. Our products undergo rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure compliance with research-grade specifications and consistent performance in sensitive enzymatic assays. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and related substance profiles is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to moisture, heat, or light should be avoided to maintain stability and performance. Allow the product to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/IR)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Assay (HPLC)	≥ 98.0% (on dried basis)
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.